Microscope Function and Slide Preparation

The video tutorial demonstrates how to prepare an onion cell slide for microscopic examination. It covers the steps of removing a thin tissue layer from an onion, staining it with iodine, and applying a coverslip to avoid air bubbles. The tutorial also explains how to set up and focus a microscope, starting with a low-power objective lens and progressing to higher magnification for detailed observation of the cells.

What is the first step in preparing an onion cell slide?

• Applying a stain

• Removing the onion skin

• Gathering a clean glass slide

• Using a coverslip

What is the purpose of removing the thin layer of tissue from the onion?

• To make the onion taste better

• To ensure the tissue lays flat on the slide

• To add color to the slide

• To increase the thickness of the sample

Why is iodine used in the preparation of the onion cell slide?

• To clean the slide

• To stain the plant cells for better visibility

• To protect the cells from damage

• To increase the size of the cells

What is the function of a coverslip in slide preparation?

• To magnify the cells

• To add weight to the slide

• To keep the tissue flat and prevent air bubbles

• To protect the microscope lens

Why is it important to avoid air bubbles when placing the coverslip?

• They can damage the microscope

• They can distort the image

• They can change the color of the stain

• They can increase the magnification

What is the role of the condenser lens in a microscope?

• To magnify the image

• To focus the light onto the slide

• To hold the slide in place

• To adjust the color of the light

Which lens should you start with when focusing a microscope?

• The lowest power objective lens

• The condenser lens

• The eyepiece lens

• The highest power objective lens

What is the purpose of using the coarse focus knob on a microscope?

• To bring the slide closer to the lens

• To change the objective lens

• To fine-tune the image clarity

• To adjust the light intensity

What is the total magnification achieved with a 40x objective lens and a 10x eyepiece lens?

• 100x

• 40x

• 400x

• 10x

What is the main advantage of using a higher power objective lens?

• It offers a higher magnification

• It reduces the need for staining

• It provides a wider field of view

• It increases the light intensity