Microarray Experiment Procedures

The video tutorial demonstrates a microarray experiment using an automated mixer. It covers RNA sample quality analysis, amplification, labeling, hybridization, and scanning of microarrays. The tutorial emphasizes the importance of visual demonstration due to the complexity of the equipment. It also details quality control analysis, gel matrix preparation, and sample loading for analysis using nano chips.

What is the primary goal of the microarray experiment procedure?

• To analyze protein samples

• To perform a microarray experiment using an automated mixer

• To study DNA sequences

• To develop new RNA samples

Which instrument is used to analyze the quality of RNA samples?

• Spectrophotometer

• Centrifuge

• Bioanalyzer

• Microscope

What is the next step after RNA amplification and labeling?

• RNA samples are hybridized to microarrays

• RNA samples are discarded

• RNA samples are stored

• RNA samples are sequenced

What do the results of the microarray experiment show?

• The protein synthesis rate

• The cell division process

• The differential expression of RNA transcripts

• The structure of DNA

Why is visual demonstration critical in this method?

• Because the equipment is inexpensive

• Because it requires no specialized equipment

• Because the steps are easy to learn

• Because the hybridization steps are difficult to learn

What is the first step in quality control analysis?

• RNA sequencing

• Data analysis

• Preparation of the bioanalyzer instrument

• Sample storage

How long can the filtered gel aliquots be stored at 4 degrees Celsius?

• 6 months

• 1 month

• 1 week

• 1 year

What is added to the filtered gel aliquot before centrifugation?

• Buffer solution

• RNA sample

• Dye concentrate

• Water

Where should the pipette tip be positioned when loading the gel dye mix?

• At the top of the well

• In the middle of the well

• At the very bottom of the well

• Outside the well