Thin Layer Chromatography Techniques

This video tutorial demonstrates how to separate and identify a mixture of lipids using the Thin Layer Chromatography (TLC) technique. It covers the preparation of the TLC plate, including drawing a baseline and marking positions for spotting compounds. The video explains how to spot compounds using microcapillary tubes and emphasizes the importance of handling them with care. It then guides viewers through the process of developing the TLC plate by placing it in the mobile phase and observing the solvent front. Finally, the video shows how to visualize the spots using a UV lamp and determine the Rf values of the compounds.

What is the first step in preparing a TLC plate for lipid separation?

• Draw a baseline with a soft pencil.

• Visualize the spots with a UV lamp.

• Apply the lipid samples.

• Place the plate in the mobile phase.

How should the positions be marked on the TLC plate?

• Using a soft pencil, at least one centimeter from the edge.

• Using a ruler and a pen.

• With a ballpoint pen.

• With a permanent marker.

What is the correct way to apply lipid samples onto the TLC plate?

• By pouring directly from the container.

• Using a pipette.

• With a microcapillary tube, gently and quickly.

• Using a brush.

What should you avoid doing once the TLC plate is placed in the mobile phase?

• Drawing a line at the solvent front.

• Moving the TLC jar.

• Observing the solvent front.

• Using tweezers to handle the plate.

When should the TLC plate be removed from the mobile phase?

• Once the spots are visible.

• When the solvent front is one centimeter from the top.

• After 10 minutes.

• When the solvent front reaches the top of the plate.

What tool is used to visualize the spots on the TLC plate?

• A microscope.

• A magnifying glass.

• A UV lamp.

• A flashlight.

What is the purpose of drawing around each spot after visualization?

• To make the spots larger.

• To highlight the spots for better visibility.

• To measure the distance traveled by the solvent.

• To remove excess solvent.

What can be determined after visualizing the spots on the TLC plate?

• The color of the compounds.

• The Rf values of the compounds.

• The weight of the compounds.

• The volume of the solvent used.