Plasmid DNA Isolation Techniques

Plasmid DNA Isolation Techniques

Assessment

Interactive Video

Biology

11th - 12th Grade

Practice Problem

Hard

Created by

Patricia Brown

FREE Resource

This video tutorial demonstrates how to isolate transfection-grade plasmid DNA using the PureLink High Pure XP Plasmid Mega Prep Kit. The process involves lysing cells, filtering lysate, binding DNA to a cartridge, and eluting purified DNA. Key steps include centrifugation, vacuum filtration, and washing with buffers. The entire procedure can be completed in approximately 90 minutes, resulting in high-quality plasmid DNA suitable for transfection.

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10 questions

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1.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is the primary function of the enhanced anion exchange resin in the plasmid DNA isolation process?

To precipitate proteins

To lyse the cells

To wash the DNA

To isolate transfection-grade plasmid DNA

2.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is the first step in lysing cells from an overnight culture?

Add lysis buffer

Centrifuge the cells

Add resuspension buffer

Discard the supernatant

3.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Which buffer is added immediately after the lysis buffer during the cell lysis procedure?

Elution buffer

Wash buffer

Precipitation buffer

Equilibration buffer

4.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is the purpose of the lysate filtration cartridge in the plasmid DNA isolation process?

To filter the lysate

To elute the DNA

To bind DNA

To wash the DNA

5.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is done with the flow-through after applying vacuum to the DNA binding cartridge?

It is used to elute the DNA

It is used to wash the DNA

It is collected for further analysis

It is discarded

6.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is the next step after loading the clarified lysate onto the equilibrated binding cartridge?

Add wash buffer

Apply vacuum and drain

Add elution buffer

Add isopropanol

7.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

How is the plasmid DNA eluted from the DNA binding cartridge?

By centrifugation

By adding elution buffer and applying a soft vacuum

By adding wash buffer

By adding isopropanol

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