DNA Replication Enzymes and Functions

The video tutorial provides an in-depth explanation of DNA replication enzymes and proteins. It begins with a recap of DNA replication in prokaryotes, then delves into the roles of DNA helicase, single strand binding proteins, and primase. DNA helicase unzips the DNA strands, while single strand binding proteins prevent reformation of the double helix. Primase synthesizes RNA primers necessary for DNA polymerase to elongate the DNA chain. The video concludes with a call to action for viewers to like, share, and subscribe.

What is the primary role of DNA helicase in DNA replication?

• To synthesize RNA primers

• To catalyze the formation of phosphodiester bonds

• To bind single-stranded DNA

• To unzip the DNA strands

Which enzyme in E. coli acts as a helicase?

• MCM

• DNA polymerase

• DNA B

• SSB

What provides the energy for DNA helicase to function?

• Formation of phosphodiester bonds

• Binding of single-strand binding proteins

• Synthesis of RNA primers

• Hydrolysis of ATP

What is the function of single strand binding proteins during DNA replication?

• To synthesize new DNA strands

• To prevent the formation of secondary structures

• To catalyze the hydrolysis of ATP

• To provide a 3'-OH group for DNA polymerase

How do single strand binding proteins bind to DNA?

• As monomers

• As hexamers

• As tetramers

• As dimers

What is the role of primase in DNA replication?

• To unwind the DNA helix

• To synthesize RNA primers

• To bind to single-stranded DNA

• To catalyze the formation of phosphodiester bonds

Which enzyme synthesizes the RNA primer in E. coli?

• DNA polymerase alpha

• DNA B

• DNAG

• MCM

What is the composition of DNA polymerase alpha in eukaryotes?

• Monomeric

• Dimeric

• Tetrameric

• Hexameric

Which subunits of DNA polymerase alpha serve as the primase?

• None

• All subunits

• One subunit

• Two subunits

What is the main function of DNA polymerase during replication?

• To elongate the DNA chain

• To bind single-stranded DNA

• To synthesize RNA primers

• To unwind the DNA helix