Microarray Technology and Applications

The video tutorial explains the microarray experiment, a technique used in genomics to study gene expression. It covers the preparation and execution of a microarray experiment, comparing cancerous and normal cells to identify active genes. The tutorial also discusses the limitations of microarray technology, emphasizing its role in detecting gene activity but not protein defects. The video concludes with a reminder of the importance of understanding molecular genetics for grasping the experiment.

What is essential to understand before grasping the microarray experiment?

• The process of cell division

• The history of genomics

• The central dogma of molecular genetics

• The structure of proteins

What does a genome represent in an organism?

• A single gene

• The protein structure

• The totality of genetic information

• Only the active genes

What is the primary purpose of a DNA microarray?

• To measure cell size

• To sequence proteins

• To identify cell types

• To detect active genes in cells

What is the first step in preparing a microarray?

• Analyzing the data

• Labeling the DNA

• Sequencing the entire genome

• Performing PCR

Why is PCR important in microarray preparation?

• It labels the DNA

• It sequences the genome

• It analyzes the data

• It amplifies gene fragments

What is used to separate mRNA from other RNA types during the experiment?

• A fluorescent dye

• A vortex mixer

• Poly-A tail binding beads

• A centrifuge

How are cancerous and healthy tissues distinguished in a microarray?

• By their weight

• By their shape

• By their size

• By their color coding with fluorescent dyes

What does a red spot on a microarray indicate?

• A gene turned off in healthy cells

• A gene turned on in cancer cells

• A gene turned off in cancer cells

• A gene turned on in healthy cells

What is a limitation of microarray technology?

• It cannot label DNA

• It cannot amplify genes

• It cannot detect proteins

• It cannot sequence DNA

What additional analysis is suggested to check for defective proteins?

• DNA sequencing

• Protein product monitoring

• Gene amplification

• Cell division analysis