Microscope Slide Preparation Techniques

Microscope Slide Preparation Techniques

Assessment

Interactive Video

Biology

9th - 10th Grade

Practice Problem

Hard

Created by

Patricia Brown

FREE Resource

The video tutorial explains the process of preparing microscope slides using flower buds. It covers the steps of fixation, embedding, sectioning, and staining. The fixation process involves preserving tissue with a chemical fixative, FAA. Embedding uses paraffin wax to encase the tissue, followed by sectioning with a microtome to create thin slices. Staining with Toluidine Blue enhances visibility of the sections, which are then observed under a microscope to reveal detailed structures of the flower bud.

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10 questions

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1.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is the primary purpose of using microscope slides in biology?

To enhance the size of specimens

To observe and record microscopic details

To store biological samples

To protect specimens from decay

2.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Which of the following is NOT a step in the general process of making microscope slides?

Staining

Magnification

Embedding

Fixation

3.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is the role of FAA in the fixation process?

To section the tissue

To embed the tissue

To preserve the tissue

To stain the tissue

4.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Why is paraffin wax used in the embedding process?

To stain the tissue

To preserve the tissue

To support the tissue for sectioning

To magnify the tissue

5.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is the function of a microtome in the sectioning process?

To fix the tissue

To embed the tissue in wax

To slice the tissue into thin sections

To stain the tissue

6.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is the purpose of staining in microscope slide preparation?

To embed the tissue

To preserve the tissue

To section the tissue

To enhance visibility of tissue structures

7.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Which stain is used in the video to color the plant tissue?

Hematoxylin

Eosin

Toluidine Blue

Crystal Violet

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