Microscope Slide Preparation Techniques

The video tutorial explains the process of preparing microscope slides using flower buds. It covers the steps of fixation, embedding, sectioning, and staining. The fixation process involves preserving tissue with a chemical fixative, FAA. Embedding uses paraffin wax to encase the tissue, followed by sectioning with a microtome to create thin slices. Staining with Toluidine Blue enhances visibility of the sections, which are then observed under a microscope to reveal detailed structures of the flower bud.

What is the primary purpose of using microscope slides in biology?

• To enhance the size of specimens

• To observe and record microscopic details

• To store biological samples

• To protect specimens from decay

Which of the following is NOT a step in the general process of making microscope slides?

• Staining

• Magnification

• Embedding

• Fixation

What is the role of FAA in the fixation process?

• To section the tissue

• To embed the tissue

• To preserve the tissue

• To stain the tissue

Why is paraffin wax used in the embedding process?

• To stain the tissue

• To preserve the tissue

• To support the tissue for sectioning

• To magnify the tissue

What is the function of a microtome in the sectioning process?

• To fix the tissue

• To embed the tissue in wax

• To slice the tissue into thin sections

• To stain the tissue

What is the purpose of staining in microscope slide preparation?

• To embed the tissue

• To preserve the tissue

• To section the tissue

• To enhance visibility of tissue structures

Which stain is used in the video to color the plant tissue?

• Hematoxylin

• Eosin

• Toluidine Blue

• Crystal Violet

What color does Toluidine Blue stain the nuclei of cells?

• Yellow

• Blue

• Red

• Green

What is the appearance of xylem cells when stained with Toluidine Blue?

• They appear yellow and elongated

• They appear blue and flat

• They appear red and round

• They appear green with a slinky shape

What is the final step in the preparation of microscope slides?

• Embedding the tissue

• Fixing the tissue

• Applying a coverslip

• Sectioning the tissue