Primer Design for Cloning ENTIRE Gene Sequence

Primer Design for Cloning ENTIRE Gene Sequence

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Science, Biology, Engineering

University

Hard

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The video tutorial explains how to design forward and reverse primers for gene cloning. It covers the process of obtaining the entire gene sequence, designing primers to amplify the full gene, and ordering them online. The forward primer is designed from the 5' end, while the reverse primer requires a reverse complement of the 3' end. The tutorial also provides guidance on using online tools for primer ordering.

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OPEN ENDED QUESTION

3 mins • 1 pt

What new insight or understanding did you gain from this video?

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