Affinity Constants

The video tutorial explains the concept of hemoglobin binding with oxygen and introduces the equilibrium reaction between hemoglobin and oxygen. It defines Theta as a measure of protein saturation and discusses equilibrium constants, affinity, and dissociation constants. The tutorial provides methods to calculate protein-ligand concentration using measurable variables and explores the relationship between affinity and dissociation constants. Practical applications of these concepts are also discussed.

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