Microbiology: Indole Test (Tryptophanase)

The video tutorial explains the indole test, a method used to identify gram-negative bacilli. It details the role of tryptophan and the enzyme tryptophanase in producing indole, which reacts with Kovacs reagent to produce a red color, indicating a positive result. The absence of this color suggests a negative result, meaning tryptophanase is not expressed. The tutorial provides a clear explanation of how to interpret the test results, emphasizing the simplicity and straightforward nature of the indole test.

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