UV-Vis Spectroscopy: Theory and "How do I know when to use it?"

UV-Vis Spectroscopy: Theory and "How do I know when to use it?"

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Science, Physics, Chemistry

University

Hard

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The video tutorial covers the basics of UV VIS spectroscopy, focusing on the absorption properties of various molecules. It explains the significance of double bonds and conjugation in determining a molecule's ability to absorb UV light. The tutorial also delves into electron transitions, particularly π to π star transitions, and their role in spectroscopy. Additionally, it outlines the UV VIS spectroscopy technique, including the use of a cuvet and light source, and discusses the relationship between energy and wavelength. The video concludes with potential applications in biochemistry, such as identifying proteins and nucleic acids.

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OPEN ENDED QUESTION

3 mins • 1 pt

What new insight or understanding did you gain from this video?

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