Microbiology: Phenylalanine Deaminase Test

The phenylalanine deaminase test is a biochemical test used to identify bacteria in the Enterobacteriaceae family, specifically the genera Proteus and Providencia. The test detects the enzyme phenylalanine deaminase, which converts phenylalanine into phenyl pyruvic acid. This acid reacts with ferric chloride, resulting in a green color change, indicating a positive result. A lack of color change indicates a negative result. The video explains the test's mechanism, detection method, and interpretation of results, concluding with a demonstration.

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