Creating a Pour Plate

What is the first step in creating a pour plate?

• Heating the agar to 100 degrees Celsius

• Adding drops of culture into a sterile petri dish

• Mixing the culture with water

• Pouring molten agar into a petri dish

At what temperature is the molten nutrient agar kept before pouring?

• 37 degrees Celsius

• 50 degrees Celsius

• 25 degrees Celsius

• 100 degrees Celsius

What is the purpose of gently shaking the petri dish after pouring the agar?

• To mix the culture with the agar

• To cool down the agar quickly

• To evenly disperse the agar through the medium

• To remove air bubbles

Why is it important to flame the neck of the bottle during the process?

• To heat the contents

• To sterilize the bottle

• To mix the contents thoroughly

• To prevent the agar from solidifying

What indicates that the pour plate is ready for use?

• The petri dish is warm to touch

• The agar has set

• The E. coli is visible on the surface

• The agar is still liquid