Tissue Culture Quiz

What is the primary significance of tissue culture in biotechnology?

• It allows for the cloning of animals.

• It helps in the synthesis of new chemical compounds.

• It is used for the production of artificial organs.

• It enables the independent development of plant cells into whole plants.

Who was the first to grow isolated plant cells on an artificial medium?

• Hibalant

• Charles Darwin

• Gregor Mendel

• Louis Pasteur

Which type of culture involves growing an isolated or immature embryo?

• Embryo culture

• Organ culture

• Suspension culture

• Tissue culture

What is essential for maintaining aseptic conditions in a tissue culture laboratory?

• High humidity

• Natural sunlight

• Sterilized air and equipment

• Open windows

Which chemical is NOT typically used for sterilization in tissue culture?

• Cresol

• Sodium chloride

• Mercury chloride

• Ethanol

What is the purpose of adding agar to the culture medium?

• To increase the pH

• To solidify the medium

• To sterilize the medium

• To provide nutrients

What is the role of cytokinins in tissue culture?

• They encourage shoot formation.

• They promote root formation.

• They inhibit cell growth.

• They prevent disease.

What is the first step in transferring plant tissues to a culture container?

• Adding growth regulators

• Adjusting the pH

• Wrapping in paper

• Surface sterilization

What was observed in carrot culture experiments?

• Carrot cells developed into whole plants.

• Carrot roots failed to grow.

• Carrot cells did not respond to cytokinins.

• Carrot cells only formed callus.

What is a significant benefit of regenerating plants from callus or suspension culture?

• Uniform plant size

• Faster growth rates

• Increased resistance to all diseases

• Enhanced genetic variability