BCA Protein Assay Quiz

What is one key advantage of the BCA protein assay over the Bradford test?

• It is faster to perform.

• It is more sensitive to protein concentration.

• It is compatible with detergents.

• It requires less sample volume.

What is the main component of Reagent A in the BCA assay?

• Hydrochloric acid

• Cupric sulfate

• Carbonate buffer with high pH

• Sodium chloride

What color change indicates the presence of protein in the BCA assay?

• Blue to green

• Green to violet

• Yellow to red

• Red to blue

During the BCA assay, what happens to copper ions in the presence of protein?

• Copper ions are reduced.

• Copper ions are neutralized.

• Copper ions remain unchanged.

• Copper ions are oxidized.

What is the purpose of using a standard curve in the BCA assay?

• To measure the pH level

• To determine the protein concentration

• To calibrate the plate reader

• To identify the protein type