Affinity Chromatography Quiz

What is the primary purpose of affinity chromatography?

• To purify a specific molecule from a complex mixture

• To mix different molecules

• To increase the temperature of a solution

• To degrade unwanted proteins

Which of the following is a type of interaction used in affinity chromatography?

• Antigen-antibody interaction

• Van der Waals forces

• Covalent bonding

• Ionic bonding

In affinity chromatography, what is the role of the receptor with a strong affinity for the protein of interest?

• To act as a mobile phase

• To dissolve the protein

• To change the color of the solution

• To immobilize the protein on the solid phase

What happens to biomolecules with no affinity for the receptor during the chromatography process?

• They bind to the stationary phase

• They travel through the column

• They are degraded

• They change color

What is the purpose of the elution buffer in affinity chromatography?

• To bind the target protein

• To wash away the target protein

• To detach the protein of interest from the receptor

• To increase the temperature of the column