Understanding Bisulfite Sequencing and Epigenetics

What is the main reason two organisms with identical DNA sequences can have different phenotypes?

• Environmental factors

• Different DNA sequences

• Epigenetic patterns

• Random mutations

In the context of DNA methylation, what is the usual consequence of cytosine methylation in the promoter region of a gene?

• Duplication of the gene

• Inactivation of the gene

• Activation of the gene

• Deletion of the gene

During bisulfite conversion, what happens to unmethylated cytosines?

• They are converted to guanine

• They remain unchanged

• They are converted to uracil

• They are converted to adenine

What is the final nucleotide that unmethylated cytosines are converted to after PCR in bisulfite sequencing?

• Guanine

• Adenine

• Cytosine

• Thymine

How can bisulfite sequencing be useful in cancer research?

• By sequencing RNA

• By identifying active oncogenes

• By detecting silenced tumor suppressor genes

• By mapping genetic mutations