Yeast Two-Hybrid System Quiz

What is the primary purpose of the yeast two-hybrid system?

• To express all genes in yeast

• To determine DNA sequences

• To identify protein-protein interactions

• To synthesize RNA

In the yeast two-hybrid system, what happens if proteins X and Y do not interact?

• The reporter gene is expressed

• The activator domain binds to the DNA

• RNA polymerase is recruited

• There is no expression of the reporter gene

What role does the activator domain play in the yeast two-hybrid system?

• It synthesizes proteins

• It recruits RNA polymerase

• It binds to the DNA

• It degrades RNA

In the lab example, what is the function of the HIS3 gene?

• It is a bait protein

• It is a transcription factor

• It is a prey protein

• It is a reporter gene for histidine biosynthesis

What indicates that proteins X and Y interact in the lab example?

• Increased DNA binding

• Yeast growth on histidine-depleted media

• No yeast growth on the plate

• Decreased RNA synthesis