Microscopy Techniques Quiz

What is one key advantage of using a hand microtome over a traditional benchtop microtome?

• It is heavier and less portable.

• It is more expensive and complex.

• It can produce thin sections quickly without pre-treatment.

• It requires chemical processing of specimens.

What material is commonly used for the top sectioning platform of a hand microtome?

• Stainless steel

• Wood

• Plastic

• Rubber

How is the thickness of sections adjusted in a hand microtome?

• By changing the blade

• Using a rotatable knob with graduations

• Using a digital display

• By altering the specimen size

Which material can be used as a pith material for embedding specimens in a hand microtome?

• Carrot

• Glass

• Metal

• Plastic

What is the purpose of using paraffin wax in specimen positioning?

• To increase the specimen's size

• To support and stabilize the specimen

• To make the specimen heavier

• To add color to the specimen

What is the recommended angle for the razor blade during sectioning?

• 20 degrees

• 5 degrees

• 15 degrees

• 10 degrees

Why should forceps or pins with sharp tips be avoided when handling sections?

• They are difficult to clean

• They are not precise enough

• They can damage the sections

• They are too expensive

What should be used to pick out good sections from the petri dish?

• A soft paint brush

• A sharp knife

• A metal spatula

• A pair of scissors

What is the final step after obtaining ideal sections using a hand microtome?

• Discarding the sections

• Making a temporary or permanent slide mount

• Re-cutting the sections

• Storing the sections in a freezer

What is the main focus of the video tutorial?

• Chemical processing of specimens

• Freehand section cutting techniques

• Advanced microscopy techniques

• Use and techniques of the hand microtome