Trizol RNA Extraction Quiz

What is the primary goal of the Trizol RNA extraction process?

• To isolate proteins from cells or tissues

• To isolate total RNA from cells or tissues

• To isolate DNA from cells or tissues

• To isolate lipids from cells or tissues

What is the purpose of adding chloroform to the Trizol mixture?

• To separate the layers during centrifugation

• To neutralize the solution

• To increase the temperature

• To dissolve the RNA

Which layer contains the RNA after centrifugation?

• The bottom organic layer

• The middle separating layer

• The top aqueous layer

• None of the layers

What is added to the aqueous solution to precipitate the RNA?

• Ethanol

• Chloroform

• Isopropyl alcohol

• Water

What should be used to resuspend the RNA pellet?

• RNase-free water

• Chloroform

• Trizol

• Ethanol