What is the primary purpose of the NEBNext Ultra II Directional RNA Library Prep Kit?

• To enrich ribosomal RNA

• To produce high-quality RNA libraries

• To sequence DNA directly

• To synthesize proteins

Which method is NOT used for ribosomal RNA removal in the library prep process?

• None of the above

• Ribosomal RNA depletion

• Poly(A) mRNA enrichment

• DNA fragmentation

What is the role of Actinomycin D during the cDNA synthesis process?

• To enhance RNA fragmentation

• To prevent second strand synthesis

• To incorporate uracils

• To ligate adaptors

What is the significance of incorporating uracils into the second strand of cDNA?

• It prevents RNA degradation

• It allows for strand-specificity

• It enhances the yield of the library

• It facilitates adaptor ligation

What is the purpose of PCR amplification in the final steps of library preparation?

• To increase library yield and incorporate barcodes

• To fragment RNA

• To remove uracil-containing strands

• To enrich ribosomal RNA