By Simon Dowle
10 questions
6th - 6th Grade
Science
606 plays
Updated Jan 2026
The Osmosis and Diffusion worksheet is a Grade 6 Biology resource with 10 multiple-choice questions focused on how water and substances move across cell membranes. Students identify osmosis as the movement of water and a form of passive transport, define diffusion as spreading out, and distinguish isotonic, hypotonic, and hypertonic solutions by comparing them with the inside of a cell. The questions also ask students to predict whether water will move into a cell, out of a cell, or back and forth when solutions have different tonicities. Completing this free printable worksheet helps students connect tonicity vocabulary to water movement and recognize equilibrium when tonicity is the same on both sides of a membrane. It includes a complete answer key for efficient review, independent practice, or assessment.
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WorksheetsAnswer KeyWater
...have the same tonicity as inside the cell.
...have a lower tonicity than inside the cell.
...have a higher tonicity than inside the cell.
...spread out.
...passive transport.
...diffuse out of the cell.
...diffuse back and forth to and from the cell.
...diffuse into the cell.
...equilibrium
This Grade 6 Biology worksheet uses 10 multiple-choice questions to check whether students can identify osmosis as water moving across a membrane and as passive transport, define diffusion as spreading out, and classify isotonic, hypotonic, and hypertonic solutions. Students then apply those definitions to predict whether water moves into a cell, out of a cell, or back and forth, and connect equal tonicity with equilibrium.
Introduce the worksheet by reviewing the meaning of diffusion, osmosis, and passive transport, then have students compare each solution type with the inside of a cell. Before students answer the directional questions, ask them to explain how hypertonic, isotonic, and hypotonic conditions should affect water movement; use the multiple-choice responses to discuss why water moves out, moves in, or moves in both directions.
Students may reverse hypotonic and hypertonic, leading them to choose water moving into the cell when the solution is hypertonic or out when it is hypotonic. They may also confuse isotonic conditions with no movement instead of the worksheet’s answer that water diffuses back and forth, or mistake diffusion for active transport rather than passive transport.
You can assign this worksheet as a printable PDF or as a digital quiz on Wayground, and it includes a complete answer key for checking the 10 multiple-choice responses. For printable submissions, scan student work with the Wayground for Teachers app to help grade it efficiently.
Use the worksheet’s Advanced Settings to adjust font spacing or increase the font size so the 10 multiple-choice items are easier to read. You can also enable a dyslexia-friendly font or translate the osmosis, diffusion, and tonicity questions into another language when that better supports access to the content.
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