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Forensics: DNA

Total questions: 47

Worksheet time: 1hrs 14mins

Name
Class
Date
1.
DNA in blood comes from:
a)
white blood cells
b)
platelets
c)
plasma
d)
red blood cells
2.
The structure of DNA is a:
a)
triple helix
b)
double helix
c)
single helix
d)
what the heck is a helix
3.
The male determines the sex of the child
a)
True
b)
False
c)
sometimes
4.
Which of the following are not sources of DNA?
a)
Blood
b)
Semen
c)
Hair
d)
Clothes
5.
What does CODIS stand for?
a)
Criminal Offense DNA Index System
b)
Criminal Offensive DNA Index Securities
c)
Combined DNA Index Securities
d)
Combined DNA Index System 
6.
Adenine (A) pairs with Guanine (G), Cytosine (C) pairs with Thymine (T)
a)
True
b)
False
7.
How many STR markers/loci does the FBI use to identify criminals in America?
a)
17
b)
4
c)
26
d)
13
8.
For vWA marker/loci, Mom is 12,15 and Dad is 15, 17. What is a possible combination for the son?
a)
12, 12
b)
13,17
c)
17, 17
d)
15, 15
9.
The repeating units in a DNA molecule are called:
a)
amino acids
b)
nucleotides
c)
monosaccharides
d)
fatty acids
10.
If one side of a DNA strand is A-A-T-C-G-G-A, what would the other side be?
a)
A-A-T-C-G-G-A
b)
G-G-C-A-T-T-AG
c)
T-T-A-G-C-C-T 
d)
C-C-G-A-T-T-C
11.
Which process requires the most DNA?
a)
RFLP
b)
STR
c)
PCR
d)
Recombinant DNA
12.
One or two alternative forms of a gene is called:
a)
RNA
b)
Restrictin Enzyme
c)
karyotype
d)
allele
13.
What is the complement of the DNA Strand:   CAG
a)
CAT
b)
GACGTC
c)
GTC
d)
GAT
14.
What does PCR stand for?
a)
Poly-ambiguous chain reaction
b)
Pentahedral corrosion remnant
c)
Polymerase chain reaction
d)
Peroxide corrosion remnant
15.
The purpose of PCR
a)
cutting DNA
b)
creating RFLPs
c)
amplifying/copy DNA
d)
reversing the charge of DNA
16.
Based on these results whose blood was found in the blood stain at the crime scene?
a)
Bob
b)
Sue
c)
John
d)
Lisa
17.
Biologists need additional copies of a DNA fragment. What tool should they use?
a)
Gel Electrophoresis
b)
PCR
c)
Restriction enzymes
d)
This isn't possible
18.
Which DNA strands move the fastest through the gel when the electricity is turned on?
a)
The long strands
b)
The short strands
c)
The medium strands
d)
They all move the same
19.
Which tissue samples are used for DNA fingerprinting? 
a)
Hair with follicular tag
b)
Skin
c)
Blood 
d)
Any of the above
20.
DNA can have forensic value even if it is decades old.
a)
True 
b)
False
21.
Which suspect’s DNA matches the felon’s DNA left at the crime scene?
a)
Suspect 1
b)
Suspect 2
c)
Suspect 3
22.
What is the majority of Forensic DNA used for solving?
a)
Murder
b)
Rape
c)
Missing person
d)
All of the above
23.
Mr. Jones was killed in a car accident.  Afterwards, a former girlfriend filed a paternity suit claiming that Mr. Jones was the father of her child, and thus she was entitled to the man’s estate.  Which sample of DNA would have to be from Mr. Jones if he were the child’s father? 
a)
2
b)
3
c)
4
d)
5
24.
A boy moves to another country to live with his father.  Years later, the boy decides to move back to live with the woman who raised him.  Immigration officials suspected that the boy was the woman’s nephew, not her son, and therefore not legally entitled to residency.  Based on the DNA fragments, is it possible that she is the boy’s mother?
a)
Yes
b)
No
25.
You load some DNA into a gel with the following Restriction Fragment Length Polymorphism lengths—25bp, 13bp, 54bp, and 11bp. Which of these four would travel the greatest distance from the well during electrophoresis?
a)
25bp
b)
13bp
c)
54bp
d)
11bp
26.
Put the following in order from 1st step to last step.
A. Stain the gel
B. PCR
C. Load the DNA
D. Analyze
E. Add restriction enzymes
F. Electrophorese
a)
B, E, C, F, A, D
b)
D, A, C, B, F, E
c)
F, C, A, E, B, D
d)
B, C, E, A, D, F
27.
How do scientists cut DNA into smaller strands?
a)
restriction enzymes
b)
scissors
c)
ligase
d)
agarose
28.
Why do you use a new pipette tip on the micropipette every time you get a new sample?
a)
To avoid contaminating the other samples
b)
To use as many tools as you can.
c)
The pipette tips disappear after being used.
d)
None of these reasons
29.
Pattern of DNA fragments obtained by examining a person’s unique sequence of DNA base pairs
a)
Chromosome
b)
DNA Probe
c)
DNA Fingerprint
d)
Electrophoresis
30.
Most human cells have how many chromosomes 
a)
23
b)
24
c)
46
d)
48
31.
DNA is found in what two organelles  
a)
Nucleus and Ribosomes
b)
Ribosomes and Mitochondria 
c)
Nucleolus and Golgi Body
d)
Nucleus and Mitochondria
32.
Mitochondrial DNA is different from nuclear DNA because
a)
it is inherited only from the mother
b)
It is inherited only from the father
c)
It is double helix in shape
d)
It is not useful in forensic science
33.
During PCR, the temperature is increased to separate DNA strands, this step is called: 
a)
Extension
b)
Denaturation
c)
Annealing
d)
Contamination
34.
During PCR, the temperature is decreased to allow primers to base pair with the complementary DNA template.  This step is called: 
a)
Denaturation
b)
Annealing
c)
Extension
d)
Contamination
35.
Y-STR analysis:
a)
is used differentiate among male profiles in a mixed sample
b)
is inherited from the mother
c)
is the same for everyone in the family
d)
all of these
36.
If a sample gives an incomplete profile, what is the most likely result?
a)
exclusion
b)
inconclusive
c)
match
37.
mtDNA :
a)
can match to an individual
b)
is circular in shape
c)
is easier to sequence
d)
all of these
38.
a)
nuclear DNA inheritance pattern
b)
Y-STR inheritance pattern
c)
mtDNA inheritance pattern
39.
a)
nuclear DNA inheritance pattern
b)
Y-STR inheritance pattern
c)
mtDNA inheritance pattern
40.
In this technique, a laser strikes each fragment as it travels through, then is directed through a prism to the correct "color", then loaded as data into a computer as peaks with numbers
a)
capillary electrophoresis
b)
gel electrophoresis
c)
PCR
d)
Extraction
41.
In this technique, DNA is loaded via a micropipettor, electric current turned on, then travels down the agarose, is stained and x-rayed.
a)
capillary electrophoresis
b)
gel electrophoresis
c)
PCR
d)
extraction
42.
Locus        Alleged Father       Child 
D2S1338       12, 13                  8, 9 
D8S1179        21.2, 32             19, 21.2
D10S1008      7, 12                 15, 18 
D19S433       15, 18                12, 15 
Based on this data, which is true?
a)
The alleged father is excluded
b)
The alleged father is NOT excluded
c)
I have not idea who the father is
d)
The results are inconclusive
43.
DNA samples must be packaged:
a)
in paper
b)
in small cardboard box
c)
must have biohazard label
d)
all of these
44.
The easiest and most convenient way to collect DNA from a suspect is:
a)
buccal swab
b)
nasal swab
c)
urine
d)
blood
45.
Why does DNA move through an electrophoresis once the electricity is passed through it?
a)
it creates a vacuum
b)
like charges attract
c)
DNA is positively charged
d)
DNA is negatively charged
46.
a)
results of gel electrophoresis
b)
results of capillary electrophoresis
c)
PCR results
d)
all of the above
47.
a)
results of gel electrophoresis
b)
results of capillary electrophoresis
c)
PCR results
d)
all of these above