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DNA Technology

Total questions: 15

Worksheet time: 8mins

Name
Class
Date
1.
Describe the bacteria phenotype in the +Plasmid  AMP/LB plate.
a)
The bacteria grew but did not glow in the dark
b)
The bacteria grew AND glowed in the dark
c)
The bacteria did not grow at all
2.
Describe the bacteria phenotype in the -Plasmid  LB plate.
a)
The bacteria grew but did not glow in the dark
b)
The bacteria grew AND glowed in the dark
c)
The bacteria did not grow at all
3.
Describe the bacteria phenotype in the -Plasmid  LB/AMP plate.
a)
The bacteria grew but did not glow in the dark
b)
The bacteria grew AND glowed in the dark
c)
The bacteria did not grow at all
4.
What ingredient in the agar causes the bacteria to fluoresce (glow in the dark)?
a)
LB
b)
Arabinose
c)
Ampicillin
d)
Agar
5.
E. Coli rely on which method of gene control (which results in glowing in the dark)?
a)
Negative
b)
Positive
6.
What is the purpose of calcium in the bacteria transformation lab?
a)
It causes the plasmid DNA to be taken into the bacteria cells
b)
It binds to the negative charges on the phosphate heads in order to neutralize charge.
7.

Definition: Purposefully adding or removing genetic material (usually in a very early embryo)

a)

Genetic Engineering

b)

GMO

c)

Restriction Enzyme

d)

Gel Electrophoresis

8.

Inserting an insulin gene into a bacterial plasmid is an example of:

a)

Gel Electrophoresis

b)

Recombinant DNA

c)

Some voodoo magic

d)

Restriction mapping

9.

The technique used to amplify DNA is called ______

a)

Recombinant DNA

b)

Genetic Engineering

c)

Polymerase Chain Reaction (PCR)

d)

Gene splicing

10.

The picture shows a ___________.

a)

Bacterium

b)

Bacteriophage

c)

HIV virus

d)

Ebola

11.

Which of the following is a reproductive method of bacteria?

a)

Lytic Cycle

b)

Lysogenic Cycle

c)

Conjugation

d)

Transformation

12.

DNA has a slight ___________ charge.

a)

Positive

b)

Negative

13.

Asexual reproduction in bacteria is called:

a)

Conjugation

b)

Binary Fission

c)

Lytic Cycle

d)

Lysogenic Cycle

14.

What kind of antibiotic was used in our bacterial transformation lab?

a)

Penicillin

b)

Arabinose

c)

Ampicillin

15.

Gel Electrophoresis is a technique used to separate DNA fragments based on ________.

a)

Size

b)

Number of A's and G's

c)

Animal species

d)

Criminal suspects

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