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Chapter 9 Biotechnology Review

Total questions: 19

Worksheet time: 10mins

Name
Class
Date
1.

In the gel below which DNA fragment is the largest?

Hint: Pay attention to the direction of travel

a)

A

b)

B

c)

C

d)

D

2.

In the gel below what process is used to separate fragments?

a)

ultraviolet radiation

b)

electricity

c)

restriction enzymes

d)

PCR

3.

What is a polymerase chain reaction use for?

a)

to make many copies of DNA

b)

to separate DNA based on size

c)

to cut DNA into fragments

d)

to make restriction enzymes

4.

What does DNA fingerprinting compare?

a)

each gene making up a person's DNA

b)

the proteins DNA codes for

c)

repeating units in coding segments of DNA

d)

repeating units in noncoding segments of DNA

5.

What four things are needed in a PCR?

a)

DNA to be copied, DNA polymerase, nucleotides, helicase

b)

DNA to be copied, DNA polymerase, nucleotides, 2 primers

c)

DNA to be copied, RNA polymerase, nucleotides, ligase

d)

DNa to be copied, RNA polymerase, 2 primers, helicase

6.

Why is heat used in a PCR?

a)

to speed the process up

b)

reattaches the DNA double helix

c)

separates the original DNA strand so it can be copied

d)

adds free floating nucleotides to the new DNA strand

7.

How much DNA is made each PCR cycle?

a)

doubles

b)

quadruples

c)

splits in half

d)

maintains the same amount

8.

How long are the DNA strands that result from being treated with restriction enzymes?

a)

2-3 base pairs

b)

4-8 base pairs

c)

10-20 base pairs

d)

unlimited base pairs

9.

Which of the following is a potential adverse (negative) effect of genetic engineering?

a)

Produce medicines

b)

Save engaged species

c)

Higher crop yield

d)

Decreased genetic diversity

10.

What is B?

a)

Plasmid

b)

Foreign gene

11.

What is A?

a)

Plasmid

b)

Foreign gene

12.

What molecular tool is needed to accomplish this process?

a)

restriction enzymes

b)

scissors

c)

microarray

d)

microscope analysis

13.

Testing humans for a potential genetic disorder is an example of

a)

gene knockout

b)

transgenic studies

c)

genetic screening

d)

gene therapy

14.

Name a way that genetic engineering in plants is beneficial

a)

Decreased crop yield

b)

Decreased resistance to disease

c)

Increased resistance to disease

d)

Increased pests

15.

If I insert genes from a squid into your genome; you would be what kind of organism?

a)

transgenic

b)

recombinant

c)

foreign

d)

cisternic

16.

This DNA fingerprint is of a child and his/her parents. Which fingerprint is the child?

a)

Person 1

b)

Person 2

c)

Person 3

d)

Unable to tell

17.

The computer analysis of biological data is a main component of?

a)

genomics

b)

proteomics

c)

DNA microarray

d)

bioinformatics

18.

Inserting a gene into a bacterial plasmid is an example of?

a)

gel electrophoresis

b)

gene therapy

c)

recombinant DNA

d)

genetic screening

19.

Why can transgenic organisms express foreign genes?

a)

This is false because they can't express foreign genes

b)

Because organisms are based on the same genetic code

c)

Because we can turn on/off certain genes

d)

Because we can cut out the parts that don't match