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Microbial Growth

Total questions: 13

Worksheet time: 8mins

Name
Class
Date
1.

Bacteria reproduce asexually by a process called

a)

replication

b)

meiosis

c)

mitosis

d)

binary fission

2.
In binary fission how does the DNA of the daughter cells compare to the DNA of the parent cell? 
a)
It is only half of the amount of DNA found in the parent.
b)
It is identical.
c)
It is a mix of the parent's DNA.
d)
It has some similarities and some differences.
3.

Which of these is aerotolerant anaerobe?

a)

3

b)

1

c)

5

d)

2

4.
Microorganism that requires oxygen as the final electron acceptor of the electron transport chain.Requires O2 to live
a)
obligate aerobes
b)
obligate anaerobes
c)
aerotolerant anaerobes
d)
facultative anaerobes
5.

Which enzyme catalyzes the reaction:


H2O2 --> H2O + O2

a)

superoxide dismutase

b)

oxidase

c)

peroxidase

d)

catalase

6.

Bacteria that love the cold...

a)

Thermophiles

b)

Mesophiles

c)

Psychrophiles

d)

Halophiles

7.

These are all regarding temperature except...

a)

Psychrophiles

b)

Halophiles

c)

Thermophiles

d)

Mesophiles

8.

Temperature and pH affect cell growth

because...

a)

they can speed up protein/enzyme function

b)

they affect protein/enzyme function

c)

acid fermentation

d)

they don't affect bacterial growth

9.

The data show growth of two species of bacteria cultured in different environments for 48 hours. One plate was exposed to the normal environment. A second was placed in a container with a BD GasPak EZ to help produce an anaerobic environment. Based on the data in the table, we can conclude that S. aureus is a(n)

a)

mesophile.

b)

aerobe.

c)

facultative anaerobe.

d)

facultative halophile.

10.

Are the plates and broths used in practical classes considered Defined Medium or Complex Medium?

a)

Defined Medium

b)

Complex Medium

11.

Selective Media...

a)

grow the bacteria at an extended rate due to the amount of nutrients the bactria will take in

b)

allows you to distinguish bacteria that ferment the

sugar (turns the tube yellow) from those that don’t

(don’t turn the tube yellow-stays red)

c)

Suppress unwanted microbes and encourage

desired microbes; allows only certain bacteria to

grow

d)

Media that allow you to distinguish different

microbes; distinction usually based on color

12.

A laboratory technician uses a MacConkey agar that is designed to suppress the growth of Gram-positive bacteria, and encourage the growth of Gram-negative microbes. MacConkey agar is a type of _____

a)

differential media

b)

selective media

c)

nutrient agar

d)

nutritive media

13.
George streaks a plate of medium from a broth culture.  After incubation, he sees confluent growth in all sectors.  Which of the following errors could NOT explain this result?
a)
He forgot to pick a colony from the plate
b)
 He forgot to flame the loop between sectors
c)
He picked additional culture from the plate after the first sector
d)
He crossed over the previous streak too many times at the beginning of a new sector.