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WorksheetsMid-semester Test review
Total questions: 21
Worksheet time: 11mins
What happens if you amplify too much DNA?
Split peaks
Incomplete adenylylation
Pull up
All of the above
What is the optimal phase to measure fluorescence to estimate DNA quantity using real-time PCR (qPCR)?
Linear phase
Exponential phase
Plateau
Baseline
What is the purpose of an Internal PCR control (IPC) in a qPCR assay?
Enables verification of the polymerase
Detects inhibition in a sample
Ensures the instrument is working properly
All of the above
What is the purpose of the passive reference dye in qPCR?
Adjusts for well-to-well differences
Detects inhibition
Confirms the polymerase is working
Negative control
How can you prevent DNA contamination?
Separate Pre and Post PCR rooms
Wearing gloves, hairnet, mask, etc.
UV irradiation of laboratory setup space
Aerosol-resistant pipette tips
All of the above
How are two STR loci of the same size separated during detection?
Allelic ladder
Internal lane standard
Silver stained fragments
Different Fluorescent labeled fragments
Which of the following markers are used for sex determination?
TH01
Amelogenin
CSF1PO
D78S820
DNA migrates from ________ to ________.
Negative, positive
Positive, negative
Smaller DNA fragments move _____.
Faster
Slower
True or False: DNA fragments are single-stranded when separated during CE
True
False
SYBR Green
Intercalates with double-stranded DNA
Intercalates with single-stranded DNA
Fluorescently labels one strand
Incorporates a fluorescent dye into individual dNTPs
Concerning spectral calibration
Instruments calculates a mathematical matrix based on a standard set of dyes
Deconvolve fluorescent signal contribution from each dye color
Is unique to each instrument
All of the above
Concerning pull-up?
Can be the result of too much template
Bleeding through of one spectral channel into another
Usually the result of off-scale peaks
Color deconvolution does not work properly to separate spectral overlap
All of the above
What is the unit of measurement for signal peak height?
Quantity
Relative Fluorescence Units (RFUs)
Peak height
What is an analytical threshold (AT)?
Peaks considered reliable above baseline noise
RFU value at which second allele in a true het. sample has not dropped out
Size standard peak
Always set to 50 RFUs
What is the Ct in qPCR
Cycle number
Cycle threshold
Threshold level
None of the level
Concerning qPCR, The greater the amount of starting DNA, the sooner this threshold value is reached. TRUE OR FALSE
True
False
During capillary electrophoresis, the capillary is filled with _____.
TBE
Agarose gel
Electrokinetic buffer
Polymer
During capillary electrophoresis, the DNA migrates from _____ to _____.
Anode, cathode
Cathode, anode
Most common STR repeat unit used in forensic DNA?
Trinucleotide
Tetranucleotide
Pentanucleotide
Dinucleotide
Why did CODIS core loci increase from 13 to 20?
Reduce likelihood of adventitious matches
Increase international compatibility
Increase discrimination power
All of the above
