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WorksheetsAgarose & Polyacrylamide Gel Electrophoresis
Total questions: 12
Worksheet time: 5mins
The Diagram below represents the results of a procedure that seperated the DNA in each sample. Results of gel electrophoresis of four organisms, bottom band labelled as band X. What does band X represents?
Largest fragment of DNA that travelled the slowest
Smallest fragment of DNA that travelled the slowest
Smallest fragment of DNA that travelled the fastest
Largest fragment of DNA that travelled the fastest
In preparation for an electrophoresis procedure, enzymes are added to DNA in order to
Convert the DNA into gel
Cut the DNA into fragments
Change the colour of DNA
Produce longer sections of DNA
What applications can agarose gel electrophoresis be used for?
Parental Testing
Sequence Endangered Species DNA
Criminal Investigation
All of the above
What is Ethidium Bromide?
Buffer
Dye
DNA solution
Restriction enzyme
Gel electrophoresis is used to separate DNA fragments on the basis of which property?
size
color
functions
chromosomes
Which type of gel electrophoresis has higher resolving power?
Agarose gel electrophoresis
Polyacrylamide gel electrophoresis
In an SDS-PAGE experiment, proteins are seperated on the basis of their
Negatively charged side chain
Different Isoelectric Point
Molecular Weight
Change to mass ratio
In an SDS-PAGE gel,
Proteins are denatured by SDS
Proteins have same charge to mass ratio
Smaller proteins migrate more rapidly through the gel
All of the above
