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WorksheetsL4&5 protein structure and enzymes
Total questions: 10
Worksheet time: 5mins
What method would you use to separate a protein based on charge
Electrophoresis
Gel Filtration
pI precipitation
Ammonium sulfate precipitation
What level of protein structure requires more than 1 polypeptide chain.
Primary
Secondary
Tertiary
Quaternary
What of the following are secondary structures
Active sites
Alpha helixs
Beta sheets
Gamma coils
Select all the factors that could cause protein denaturation
Heat
Organic solvents
pH extremes
Detergents
Which of these is not a function of an Enzyme
Store molecules
Bind to substrates
Catalyze reactions
Transfer organic groups
How do Enzymes speed up reactions.
Enzymes provide a higher activation energy for reactions meaning more reactants can react
Enzymes provide a lower activation energy for reactions meaning more reactants can react
Enzymes alter the charge of reactants making them more likely to react
Enzymes heat up reactants giving them more energy and making them more likely to react
What effect does heat on biological reactions
Increases the reaction velocity
Decreases the reaction velocity
Increases the reaction velocity to a point, then the reaction rate will plateau
Increases the reaction velocity to a point, then the reaction rate will plummet.
What do Ligase Enzymes do
Re-arrange functional groups
Join molecules together
Break bonds
Move functional groups
What is the enzyme model called where, the enzyme changes its shape to form an enzyme substrate complex, then reverts to its original shape.
Lock and Key Model
Induced Fit Model
Enzymes act as regulators for the production of organic molecules but enzymes are not actually regulated themselves as there naturally exist the correct amount of enzymes in the cell at all times.
True
False
