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Analytical Techniques

Total questions: 30

Worksheet time: 17mins

Name
Class
Date
1.
What is chromatography?
a)
Donald Trump (Luke's boy)
b)
It is something when something spilled on your paper.
c)
is a laboratory technique used in separating mixtures.
d)
Me.
2.
Which forms of Chromatography give qualitative and quantitative results?
I paper chromatoraphy
II TLC
III HPLC
IV GC
a)
I and II
b)
III and IV
c)
II only
d)
III only
3.

Which technique is able to separate gaseous substances into different components

a)

Thin Layer Chromatography

b)

HPLC

c)

Gas Chromatography

d)

Mass Specroscopy

4.
The mobile phase in paper chromatography is typically a
a)
plasma
b)
solid
c)
gas
d)
liquid
5.
Which forms of Chromatography use Rt values?
I paper chromatoraphy
II TLC
III HPLC
IV GC
a)
I and II
b)
III and IV
c)
II only
d)
III only
6.

Which of the gases below can be used as a carrier gas for GC?

I Oxygen

II Nitrogen

III Chlorine

IV Argon

a)

I and II

b)

III and IV

c)

II and IV

d)

IV only

7.

Separation in GC occurs in the ____

a)

Gas tank

b)

Oven

c)

Detector

d)

Column

8.

A pure substance shows ------------------ spot on chromatogram

a)

0

b)

1

c)

2

d)

3

9.
The retention factor is used
a)
to zero the chromatography scale
b)
to identify unknown compounds in a sample
c)
to retain solvent
d)
as a starting tool for chromatography
10.
A high Rf value indicates strong
a)
strong affinity to the stationary phase
b)
strong affinity to the mobile phase
c)
no affinity to the stationary phase
d)
 no affinity to the stationary phase
11.
A low retention time indicates
a)
a high affinity to the mobile phase
b)
a low affinity to the stationary phase
c)
a high solubility in the mobile phase
d)
all of the above
12.

Proteins can be identified and quantified by using HPLC by comparison with known standards.

The concentration of the fatty acids can be determined by measuring

a)

their retention times

b)

the temperature of the column

c)

the flow rate of the mobile phase

d)

the area under each of the peaks

13.
On the chromatogram given -------------- is mixture.
a)
red
b)
orange
c)
green
d)
black
14.

The diagram below shows a chromatogram obtained when a sample X was analysed together with four other known dyes P, Q, R and S. Dye Q was known to cause cancer. Which of the following dye(s) is/are safe for use?

a)

P only

b)

S only

c)

P and S

d)

P and X

15.
The Rf value  for the blue component is 
a)
0.3
b)
0.7
c)
10
d)
3
16.
The Rf value  for the red component is 
a)
0.3
b)
0.7
c)
10
d)
7
17.
In Chromatography the retardation factor Rf are used for identification of components. A high  Rf and a low Rt (retention time) mean the component has a 
a)
greater affinity to the stationary phase in both TLC and HPLC.
b)
greater affinity to the stationary phase in TLC but a greater affinity for the mobile phase in HPLC
c)
greater affinity to the mobile phase in TLC but a greater affininty for the stationary phase in HPLC
d)
greater affinity to the mobile phase in  both TLC  and HPLC
18.
In Chromatography the retardation factor Rf are used for identification of components. A low  Rf and a high Rt (retention time) mean the component has a 
a)
greater affinity to the stationary phase in both TLC and HPLC.
b)
greater affinity to the stationary phase in TLC but a greater affinity for the mobile phase in HPLC
c)
greater affinity to the mobile phase in TLC but a greater affininty for the stationary phase in HPLC
d)
greater affinity to the mobile phase in  both TLC  and HPLC
19.
A chromatography experiment uses water as a mobile phase. Which of the following would have the highest Rf value
a)
ethanol
b)
propanol
c)
butanol
d)
pentanol
20.
A student extracts the colour from some green smarties and uses paper chromatography to separate components colours. She finds blue has a Rf =0.38 and yellow Rf =0.75 this suggests 
a)
the yellow is more strongly adsorbed onto the stationary phase and is less solublein the mobile phase.
b)
the yellow is more strongly adsorbed onto the stationary phase and is more soluble in the mobile phase.
c)
the blue is more strongly adsorbed onto the stationary phase and is less solublein the mobile phase.
d)
the blue is more strongly adsorbed onto the stationary phase and is less solublein the mobile phase.
21.
In order to compare to chromatograms what must be kept the same?
a)
stationary  phase
b)
mobile phase
c)
temperature
d)
all of the above
22.
Which of the following factors would not affect an Rf value
a)
solvent used
b)
time taken
c)
temperature
d)
paper used
23.

A sample of caffeine has a retention time of 18.0 min on a HPLC. Which of the following changes in conditions would increase the retention time?

a)

Increasing the operating temperature.

b)

Decreasing the eluent flow rate

c)

Increasing the concentration caffeine in the sample

d)

Decreasing the concentration of caffeine in the sample

24.
Which instrument is used to measure and record the absorption spectrum of a chemical substance?
a)
Laser
b)
TLC
c)
Mass spectrograph
d)
Spectrophotometer
25.

Which instrumental technique allows you to determine the concentration of a solution?

a)

Paper chromatography

b)

Mass spectroscopy

c)

UV-Vis spectroscopy

d)

Infrared spectroscopy

26.

Based on the colour wheel, if a compound absorbs yellow light, what colour will it appear as?

a)

Red

b)

Green

c)

Purple

d)

Yellow

27.

The function of the monochromator in a UV Visible spectrophotometer is to

a)

detect the transmitted light

b)

detect the absorbed light

c)

produce light of a range of wavelengths

d)

select the wavelength of light

28.

The graph shows absorption spectra of three food dyes: Blue No. 1, Red No. 2 and Yellow No. 4.

Which one of the following is the best wavelength to determine the concentration of Red No. 2 dye in a solution containing only Red No. 2?

a)

400 nm

b)

500 nm

c)

530 nm

d)

600 nm

29.

The graph shows absorption spectra of three food dyes: Blue No. 1, Red No. 2 and Yellow No. 4.

Which one of the following is the best wavelength to determine the concentration of Red No. 2 dye in a solution containing a mixture of all three dyes?

a)

400 nm

b)

500 nm

c)

530 nm

d)

620 nm

30.

Which is the correct order of steps to determine the concentration of a solution using UV-Vis spectroscopy?

I Determine absorption maxima

II Plot calibration curve

III Measure absorbance

IV Prepare standard solutions and blank

a)

I, II, III, IV

b)

II, III, IV, I

c)

I, IV, III, II

d)

IV, III, II, I

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