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BME 221 F2019 Exam 1 Review

Total questions: 10

Worksheet time: 30mins

Name
Class
Date
1.

All of the following are true of "melting" or "denaturing" DNA during PCR EXCEPT?

a)

The hydrogen bonds are disrupted

b)

The phosphodiester linkage is disrupted

c)

The double helical structure is lost

d)

Occurs as a result of temperature changes

e)

All of these are true

2.

The critical micelle concentration refers to

a)

The minimum concentration of lipid molecules required to form micelles

b)

The maximum concentration of lipid molecules required to form micelles

c)

The minimum concentration of lipid molecules soluble in a polar solvent

d)

The concentration above which micelles can no longer form

e)

The concentration above which multilamellar vesicles form over unilamellar ones

3.

Common secondary structures are energetically favored because

a)

A. They optimize the backbone hydrogen bonds

b)

B. They optimize the adjacent residue hydrogen bonds

c)

C. They minimize clashes between adjacent groups

d)

D. A and C

e)

E. A, B, and C

4.

In a conservative substitution or replacement

a)

A frame shift has occurred leading to a new amino acid

b)

A large segment of DNA was deleted

c)

The new amino acid has similar biochemical properties to the original

d)

The new amino acid has different biochemical properties to the original

e)

The protein is exactly the same

5.

Which of the following statements about alpha helices is FALSE?

a)

The helix is typically right handed

b)

Each residue in the helix creates a 100 degree turn of the helix

c)

The backbone atoms are in the core of the helix

d)

The helix has an overall macrodipole, where the C-terminal is partially positively charged and the N-terminal is partially negatively charged

e)

The hydrogen bonds holding the helix together are parallel to the helix axis

6.

Because some proteins fold with the assistance of molecular chaperones, the thermodynamic hypothesis does not apply to these proteins.

a)

TRUE

b)

FALSE

7.

All proteins have quaternary structure.

a)

TRUE

b)

FALSE

8.

A likelihood ratio of 1 indicates that the probability of the ith amino acid being replaced by the jth amino acid is the same as due to random chance.

a)

TRUE

b)

FALSE

9.

Protein tertiary structure is generally more highly conserved than the primary structure.

a)

TRUE

b)

FALSE

10.

Of the following 15-base pair double stranded DNA sequences (note: only one strand is shown), which will be MOST stable at higher temperatures.

a)

CCCGCATCGCCATCG

b)

CATCCTAGCGACTAT

c)

AAATGCATACATCTT

d)

CGCTTATCGCCATAA