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Microbial Growth, Metabolism, and Genetics

Total questions: 15

Worksheet time: 14mins

Name
Class
Date
1.

Which group of organisms is most likely to infect humans (hint: human body temp is ~37C)

a)

psychrophiles

b)

mesophiles

c)

thermophiles

d)

hyperthermophiles

2.

Which group of organisms will use oxygen if it is present, but can survive without it?

a)

obligate aerobe

b)

obligate anaerobe

c)

facultative anaerobe

d)

aerotolerant anaerobe

3.

During which phase of growth are antibiotics most effective (because most of the organisms are dividing)?

a)

lag

b)

log/exponential

c)

stationary

d)

death

4.

Breaking large molecules into smaller molecules would be considered?

a)

anabolism

b)

catabolism

5.

Which metabolic pathway generates the most ATP?

a)

aerobic respiration

b)

anaerobic respiration

c)

fermentation

d)

glycolysis

6.

Which step of aerobic respiration generates the most ATP?

a)

glycolysis

b)

processing of pyruvate

c)

TCA cycle

d)

electron transport chain

7.

Where does the TCA cycle occur in a prokaryotic cell?

a)

mitochondria

b)

chloroplast

c)

nucleus

d)

cytoplasm

8.

Where on an enzyme does a substrate bind?

a)

active site

b)

deep pocket

c)

key hole

d)

domain of catalysis

9.

E. coli undergoes binary fission approximately every 20 minutes. If a culture started with 1000 cells, how many cells would be there be after 3 hours? (Remember Nt=Ni x 2n)

a)

64,000

b)

1,048,576,000 (1.0 x 109)

c)

512,000

d)

8000

10.

If you saw a piece of DNA that contained both introns and exons, what type of organism would you assume it came from?

a)

prokaryote

b)

eukaryote

c)

virus

d)

prion

11.

What base does cytosine pair with?

a)

adenine

b)

thymine

c)

uracil

d)

guanine

12.

What nitrogenous base is missing from RNA?

a)

adenine

b)

thymine

c)

uracil

d)

guanine

e)

cytosine

13.

Which RNA contains the message that will be translated into protein?

a)

mRNA

b)

tRNA

c)

rRNA

d)

siRNA

14.

If you were given the DNA sequence below, what would be the complementary mRNA sequence?

DNA: 5'-ATGCAT-3'

a)

3'-TAGTAC-5'

b)

3'-TACGTA-5'

c)

3'-UACGUA-5'

d)

3'-AUGCAU-5'

15.

What kind of mutation results from a normal codon becoming a stop codon as seen in the image above?

a)

missense mutation

b)

nonsense mutation

c)

stop mutation

d)

silent mutation