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WorksheetsBiotechnology
Total questions: 18
Worksheet time: 9mins
In molecular cloning, a __________ is used to insert a fragment of DNA into the host cell.
plasmid
DNA fragment
bacterium
virus
In reproductive cloning, the egg cell of one organism is used by removing the __________ nucleus and then combining the __________ nucleus from a cell from a second individual. Once the cell starts to divide, it is placed in a surrogate mother.
DNA, RNA
RNA, DNA
haploid, diploid
diploid, haploid
Organisms that have foreign DNA inserted from a different species are called
transgenic
transmutated
adenoviruses
haploid
What cell organelle carries its own DNA passed down from the mother and is used to trace genealogy?
ribosome
golgi apparatus
mitochondria
vesicles
In order to manipulate genetic material, DNA must be extracted from cells. What is the correct order of the process used to manipulate DNA?
enzymes inactivate proteins, buffering compounds break-down cell, alcohol to bring out DNA
gelatinous mass of DNA broken down using buffering compounds, alcohol to remove DNA, enzymes to inactivate proteins
buffering compounds to break-down cell, enzymes to inactivate proteins, alcohol to bring out DNA
none of the choices are correct
The process where an electric current is used to separate nucleic acids based on size and is used in forensics is called
gel electrophoresis
cloning
transgenic manipulation
PCR
The process used to make copies of DNA when not much is available and is often used in forensics is called
gel electrophoresis
cloning
PCR
transgenics
The vector used in genetic engineering is a(n)
bacterium
adenovirus
bacteriophage
transgenic donator
Polymerase Chain Reaction
Is used to make copies of specific regions of DNA
Uses a special form of DNA polymerase and primers to copy DNA
is used in crime scenes and paternity cases to compare DNA samples
All of the choices are correct
some of the choices are correct
The polymerase chain reaction is important because it allows us to
Make many copies of a targeted segment of DNA
Insert eukaryotic genes into prokaryotic plasmids
Incorporate genes into viruses
Make DNA from RNA
A desired DNA segment is inserted into small circular DNA from prokaryotes called a __________ and then is inserted into a bacterial host and the resulting combination is called ____________.
Virus, a eukaryote
Plasmid, a eukaryote
Plasmid, recombinant DNA
Virion, recombinant DNA
Proteins that suppress the transcription of a gene are called
plasmids
vectors
inducers
repressors
The term for when all of the genes needed to synthesize a particular protein are located in the same location on a chromosome.
operon
inducer
vector
repressor
When there is an over abundance of an amino acid present in a cell, a molecule will bind the the operator and RNA synthesis is blocked preventing the production of more of this amino acid. This process is called
RNA synthase
Negative regulation
Positive regulation
TRP operon
This produces multiple identical copies of a gene for basic research
restriction enzymes
gene cloning
DNA ligase
gel electrophoresis
This seals the sticky ends of restriction fragments to make recombinant DNA
restriction enzymes
gene cloning
DNA ligase
reverse transcriptase
This cuts DNA molecules at specific locations
restriction enzymes
DNA ligase
polymerase
ATP
Genetically engineered plants
are more difficult to engineer than animals
include a transgenic rice plant that could help prevent vitamin A deficiency
are being rapidly developed, but traditional plant breeding programs are still the only method to develop new plants
are banned throughout the world
