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Worksheets

Biotechnology

Total questions: 18

Worksheet time: 9mins

Name
Class
Date
1.

In molecular cloning, a __________ is used to insert a fragment of DNA into the host cell.

a)

plasmid

b)

DNA fragment

c)

bacterium

d)

virus

2.

In reproductive cloning, the egg cell of one organism is used by removing the __________ nucleus and then combining the __________ nucleus from a cell from a second individual. Once the cell starts to divide, it is placed in a surrogate mother.

a)

DNA, RNA

b)

RNA, DNA

c)

haploid, diploid

d)

diploid, haploid

3.

Organisms that have foreign DNA inserted from a different species are called

a)

transgenic

b)

transmutated

c)

adenoviruses

d)

haploid

4.

What cell organelle carries its own DNA passed down from the mother and is used to trace genealogy?

a)

ribosome

b)

golgi apparatus

c)

mitochondria

d)

vesicles

5.

In order to manipulate genetic material, DNA must be extracted from cells. What is the correct order of the process used to manipulate DNA?

a)

enzymes inactivate proteins, buffering compounds break-down cell, alcohol to bring out DNA

b)

gelatinous mass of DNA broken down using buffering compounds, alcohol to remove DNA, enzymes to inactivate proteins

c)

buffering compounds to break-down cell, enzymes to inactivate proteins, alcohol to bring out DNA

d)

none of the choices are correct

6.

The process where an electric current is used to separate nucleic acids based on size and is used in forensics is called

a)

gel electrophoresis

b)

cloning

c)

transgenic manipulation

d)

PCR

7.

The process used to make copies of DNA when not much is available and is often used in forensics is called

a)

gel electrophoresis

b)

cloning

c)

PCR

d)

transgenics

8.

The vector used in genetic engineering is a(n)

a)

bacterium

b)

adenovirus

c)

bacteriophage

d)

transgenic donator

9.

Polymerase Chain Reaction

a)

Is used to make copies of specific regions of DNA

b)

Uses a special form of DNA polymerase and primers to copy DNA

c)

is used in crime scenes and paternity cases to compare DNA samples

d)

All of the choices are correct

e)

some of the choices are correct

10.

The polymerase chain reaction is important because it allows us to

a)

Make many copies of a targeted segment of DNA

b)

Insert eukaryotic genes into prokaryotic plasmids

c)

Incorporate genes into viruses

d)

Make DNA from RNA

11.

A desired DNA segment is inserted into small circular DNA from prokaryotes called a __________ and then is inserted into a bacterial host and the resulting combination is called ____________.

a)

Virus, a eukaryote

b)

Plasmid, a eukaryote

c)

Plasmid, recombinant DNA

d)

Virion, recombinant DNA

12.

Proteins that suppress the transcription of a gene are called

a)

plasmids

b)

vectors

c)

inducers

d)

repressors

13.

The term for when all of the genes needed to synthesize a particular protein are located in the same location on a chromosome.

a)

operon

b)

inducer

c)

vector

d)

repressor

14.

When there is an over abundance of an amino acid present in a cell, a molecule will bind the the operator and RNA synthesis is blocked preventing the production of more of this amino acid. This process is called

a)

RNA synthase

b)

Negative regulation

c)

Positive regulation

d)

TRP operon

15.

This produces multiple identical copies of a gene for basic research

a)

restriction enzymes

b)

gene cloning

c)

DNA ligase

d)

gel electrophoresis

16.

This seals the sticky ends of restriction fragments to make recombinant DNA

a)

restriction enzymes

b)

gene cloning

c)

DNA ligase

d)

reverse transcriptase

17.

This cuts DNA molecules at specific locations

a)

restriction enzymes

b)

DNA ligase

c)

polymerase

d)

ATP

18.

Genetically engineered plants

a)

are more difficult to engineer than animals

b)

include a transgenic rice plant that could help prevent vitamin A deficiency

c)

are being rapidly developed, but traditional plant breeding programs are still the only method to develop new plants

d)

are banned throughout the world