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WorksheetsPharmaceutical Biotechnology
Total questions: 12
Worksheet time: 6mins
The following enzyme is used to cut nucleic acid in a specific manner:
Ligase.
Restriction endonuclease.
Reverse transcriptase.
Nuclease.
The following host cell is able to produce full activity of human protein:
E. coli.
Salmonella sp.
Bacillus sp.
Chinese hamster ovarian cells.
The procedure above is used to transfer DNA onto a solid suport from electrophosed gel:
Southern blotting.
Northern blotting.
Western blotting
Eastern blotting.
For visualization of DNA, electrophosed gel is stained with
Bromophenol blue.
Glucose.
Ethidium bromide.
Coomasie blue.
The following sequence on the plasmid DNA is used in selecting transformants.
Poly A.
Selectable marker.
Origin of replication.
Eukaryotic promoter.
The advantages of bacteria as a host cell are true EXCEPT:
It requires a low cost media.
Rapid growing host cell.
Easy to scale up.
It is able to glycosylate protein.
The following reasons explain why DNA or RNA has limited potential therapeutic applications EXCCEPT:
They are susceptible to intracellular nucleases.
They possess negative charge.
They are lipophilic in nature.
They have large molecular size.
Which of the following is more susceptible to nucleases activity?
Phosphodiester.
Phosphothioate.
Peptide nucleic acid.
Alkyl phosphonate.
The following technology targets RNA EXCEPT:
Antisense.
RNAi
Aptamer.
Ribozymes.
siRNA can be used to treat the following:
Cancers.
Viral infections.
Inflammations.
Genetic disorders.
The following limitations are the drawbacks of siRNA as a therapeutic agent EXCEPT:
Off target effects.
Poor penetrate biological membrane.
Poor pharmacokinetics property.
Extensive chemical modification to achieve satisfactory half-life.
siRNA will be accumulated mostly in the following cellular compartment after being taken up by the cell:
Cytoplasm.
Mitochondrial.
Lysosome.
Nucleus.
