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MI 1.1 Online Review 2

Total questions: 10

Worksheet time: 5mins

Name
Class
Date
1.

Which of the following cannot be determined by an ELISA test?

a)

Concentration of antigens

b)

Presence of disease

c)

Genetic sequence of a pathogen

d)

Order of infection

2.
What does ELISA stand for?
a)
E coli Immune System Antigen
b)
Enzyme-linked Immunosorbent Assay
c)
Enzyme Leuko-immune Standard Assessment
d)
Estimated Lymph Integrated System Assessment
3.

Which of the following most definitely identifies a pathogen?

a)

consensus of symptoms of those affected

b)

presence of absence of a rash

c)

response to antibiotics

d)

DNA sequencing

e)

none of these

4.

After adding the secondary antibody and enzyme complex to the ELISA, what is added next (after washing) to create the color change?

a)

substrate

b)

primary antibody

c)

enzyme #2

d)

antigen

5.

A tube is diluted at a rate of 1/4 for each consecutive tube. The concentration of tube #1 is 64ng/dL. What is the concentration of tube #4?

a)

4 ng/dL

b)

1 ng/dL

c)

0.25 ng/dL

d)

1024 ng/dL

6.
________ have surface _________ where _______ attach
a)
pathogens, antigens, antibodies
b)
antigens, antibodies, pathogens
c)
pathogens, antibodies, antigens
d)
antibodies, antigens, pathogens
7.

B lymphocytes respond to the initial antigen challenge by

a)

reducing its size

b)

immediately producing antigen-specific antibodies

c)

forming a large number of cells that are unlike the original B lymphocyte

d)

producing progeny cells that include plasma cells and memory cells

8.

What is the nucleotide sequence for the following DNA sequencing data? Choose the best answer

a)

CTTCACGCCAAGTTGTTGTGGGAGGC

b)

GTTGAGCGGAACTTCTCCCACCG

c)

GAAGTGCGGTTCAACACCCTCCG

d)

CAACTCGCCTTGAAGAGGGTGGC

9.

The search tool used to identify pathogens from a sequence of nucleotides is which of the following?

a)

ELISA

b)

BLAST

c)

DNA Sequencing

d)

PCR

10.

When analyzing your blast results, a high E- value would indicate which of the following?

a)

the match is likely accurate

b)

the match is likely inaccurate