WorksheetsPBS 1.1 Online Review 4
Total questions: 14
Worksheet time: 14mins
Name this tool (two correct answers)
Restriction enzyme
Gel electrophoresis
DNA fingerprinting
HaeIII
What is the correct order of steps for extracting DNA from cells?
1) Bundle the DNA by adding alcohol. DNA is dissolved in water but clumps together in alcohol. By adding alcohol to the purified DNA, it becomes a solid; it looks like white string. Now that it is visible, it can be easily picked up for processing
2) Clean the DNA of any proteins still attached. Histones are an example of a protein that the DNA may still be clinging to
3) Break open the cell and nucleus
4) Purify the DNA of any cell proteins, cell waste, other organelles, cell membrane components, and more
1, 2, 3, 4
3, 4, 1, 2
4, 2, 1, 3
2, 3, 1, 4
In preparation for an electrophoresis procedure, restriction enzymes are added to DNA in order to
convert the DNA into gel
cut the DNA into fragments
change the color of the DNA
produce longer sections of DNA
A student performed a gel electrophoresis experiment. The results are represented in the diagram below. Compared to the fragments at the top of the gel, the fragments at the lower end are
larger, and move slower
larger, and move faster
smaller, and move faster
smaller, and move slower
Which chemicals are used to cut DNA into fragments for a gel electrophoresis procedure?
enzymes
molecular bases
hormones
ATP molecules
This technique used to analyze DNA involves the
synthesis of new DNA strands from subunits
separation of DNA fragments based on size
production of genetically engineered DNA molecules
removal of defective genes from DNA
Gel electrophoresis is used to separate DNA fragments on the basis of which property?
size
color
functions
chromosomes
1. If the recognition sequence of the restriction enzyme HindIII is AAGCTT, then how many pieces will the DNA be broken into?
1
2
3
4
The unpaired nucleotides produced by the action of restriction enzymes are referred to as.....
sticky end.
base sequence.
single strands.
restriction fragments.
