WorksheetsProtein Characterization Pre-Test
Total questions: 12
Worksheet time: 6mins
Step for protein analysis
Protein extraction
Protein purification
Protein characterization
All of the above
True or false: Proteolytic enzymes must be inactivated during the protein extraction process
True
False
True or false: The amino acids to be determined for composition can be separated by HPLC
True
False
True or false: Determination of the amino acid sequence using the Edman degradation method
True
False
True or fasle: Longer polypeptide chains are broken into shorter ones for analysis by specifically cleaving them with enzymes that cleave at specific points.
True
False
True or false: In determining BM protein using MS the smaller ions will fly faster and press the detector first then the larger ions reach. The ions hit the detector in order by mass.
True
False
Electrophoresis used for
Separates molecules in the mixture based on solubility
Separating a molecule in a mixture under the influence of an electric field
Separates the molecules in the mixture based on molecular weight
Separates the molecules in the mixture based on the charge of ions
True or false: The principle of agarose electrophoresis is that charged particles will move towards electrodes that have opposite charges under the influence of an electric field.
True
False
True or false: The principle of electrophoresis SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrilamide Gel Reaction) protein separation techniques by migrating acrilamide components based on differences in molecular weight.
True
False
True or fasle: Western Blot (WB) is a technique for marking a protein on a nitrocellulose membrane, nylon, or other transfer membrane after the protein is separated by electrophoresis.
True
False
ELISA is one method for the detection of protein expression by principle
Antigen detection
Antibody detection
Antigen or antibody detection
Immuno assay
All of the above
True or false: SDS-PAGE is used to monitor protein purification
True
False
