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Gene Regulation

Total questions: 20

Worksheet time: 10mins

Name
Class
Date
1.

All the genes in both a prokaryotic cell and a eukaryotic cell are expressed at the same time.

a)

True

b)

False

2.

Prokaryotes regulate gene expression primarily

a)

in order to maintain homeostasis

b)

in response to changing environmental conditions

c)

during development as cells specialize

d)

Prokaryotes do not regulate gene expression. All genes are always on.

3.

One way to regulate the expression of a gene is to control the formation of mRNA. This is called ___ control.

a)

transcriptional

b)

translational

c)

post-transcriptional

d)

post-translational

4.

In order for a gene to be expressed, ___ must bind to the gene's ___, a specific sequence of nucleotides at one end of the gene, thus initiating transcription.

a)

DNA polymerase, promotor

b)

DNA polymerase, operator

c)

RNA polymerase, promotor

d)

RNA polymerase, operator

5.

An operon is

a)

a genetic on/off switch in prokaryotes

b)

a genetic on/off switch in eukaryotes

c)

a group of related genes and the associated gene switches in prokaryotes

d)

a group of related genes and the associated gene switches in eukaryotes

6.

Genes can be shut off (not expressed) when a ___ binds to the ____.

a)

activator, operator

b)

repressor, operator

c)

corepressor, operator

d)

repressor, promotor

7.

What molecule can remove a repressor protein from the operator?

a)

a corepressor

b)

an inducer

8.

What is the inducer in the lac operon?

a)

cAMP

b)

glucose

c)

lactose

d)

CRP

9.

Genes can be turned on (expressed) when a(n) ____ such as CRP or cAMP receptor protein binds to the CRP binding site.

a)

repressor

b)

corepressor

c)

inducer

d)

activator

10.

In the lac operon system, __ must first bind to CRP so that it is capable of binding to the DNA. This occurs when glucose levels are __.

a)

cAMP, high

b)

cAMP, low

c)

inducer, high

d)

inducer, low

11.

Negative control of gene expression is mediated by __ and results in a(n) __ in the frequency of transcription.

a)

activators, increase

b)

activators, decrease

c)

repressors, increase

d)

repressors, decrease

12.

In the trp operon, __ acts as a __ and helps the repressor bind to the operator.

a)

tryptophan, corepressor

b)

tryptophan, inducer

c)

lactose, corepressor

d)

lactose, inducer

13.

The trp operon has only ___ control which is common for the genes needed for ___ processes.

a)

positive, catabolic

b)

positive, anabolic

c)

negative, catabolic

d)

negative, anabolic

14.

Eukaryotes need to regulate their genes

a)

during cell specialization that occurs during development

b)

in order to maintain homeostasis

c)

both a and b

d)

neither a or b

15.

Which of the following is/are similarities between prokaryotic and eukaryotic gene expression?

a)

both use proteins to interact with the DNA to control gene expression

b)

both have histones complexed with their DNA

c)

both separate the processes of transcription and translation spatially

d)

all of the above

16.

___ are DNA sequences which are often located far from the genes they regulate in eukaryotes where transcription activators can bind.

a)

histones

b)

promotors

c)

enhancers

17.

Which of the following help to turn genes on so that they are expressed (transcribed and translated)?

a)

a high level of condensation (coiling around histones)

b)

histone acetylation

c)

DNA methylation

d)

histone methylation

18.

Inheritance NOT involving the nucleotide sequence of the DNA but involving chromatin modifications such as methylation is called

a)

epigenetic inheritance

b)

antigenetic inheritance

19.

Which of the following are types of post-transcriptional control of genes in eukaryotes?

a)

alternative RNA splicing or processing

b)

gene silencing through RNA interference

c)

destruction of ubiquitin tagged proteins

d)

all of the above

20.

Alternative RNA splicing involves

a)

removing introns and splicing the same exons together

b)

removing exons and splicing the same introns together

c)

removing introns and splicing different exons together

d)

removing exons and splicing different introns together