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Protein metabolism and enzymes

Total questions: 20

Worksheet time: 12mins

Name
Class
Date
1.

What happens when animals die

a)

Their nitrogen compounds dissipate into the air

b)

Their nitrogen compounds will return to the soil

c)

Their nitrogen compounds produce toxic gas

d)

Their nitrogen compounds combine with oxygen

2.

How animals get the nitrogen they need

a)

from the air they breathe

b)

from the water they drink

c)

from the food they eat

d)

from the Sun

3.

What is the role of denitrifying bacteria

a)

change nitrate back into nitrogen gas

b)

change nitrogen gas into nitrate

c)

absorb carbon dioxide in the soil

d)

release oxygen gas into the atmosphere

4.
Which of the following describes the role of nitrogen-fixing bacteria in the nitrogen cycle? Nitrogen-fixing bacteria convert
a)
nitrates intro nitrogen gas
b)
ammonia into proteins
c)
nitrogen gas into ammonia
d)
proteins into nitrates
5.

What is the role of Michaelis-Menten equation?

a)

To calculate the free energy

b)

Mathematically explain the relationship of enzyme's velocity and the substrate concentration

c)

Mathematically explain the saturation of enzyme in substrate

d)

To simplify Lineweaver-Burke plot

6.

Select the incorrect statement related to Michaelis-Menten plot (graph)

a)

At zero concentration of substrate, meaning [S]=0, the velocity is zero

b)

As the concentration of the substrate increases, the formation of product also increases

c)

The plot is generated using different concentration of substrate

d)

The plot is generated using different concentration of enzyme

7.

Choose the incorrect statement on Km.

a)

The unit for length is cm. The unit for Km is substrate concentration

b)

Km is the value of [S] when the velocity is half Vmax

c)

Km can be obtained directly from Lineweaver-Burke plot

d)

Km is a Michaelis-Menten constant

8.

In which type of Inhibition where the inhibitor binds to the same active site of the substrate?

a)

Uncompetitive

b)

Non competitive

c)

Competitive

d)

All of the above

9.

Which domain of an enzyme is non-protein?

a)

Active site

b)

The stabilizing body

c)

The upper most region

d)

Co-factor

10.
A catalyst works by
a)
changing the order of the reaction
b)
increasing the temperature
c)
lowering the activation energy
d)
making the activated complex
11.

If the temperature is reduced to 27 degree celcius, a reaction rate will ______

a)

increase

b)

decrease

c)

stay the same

12.

If the concentration of a reactant is increased, the reaction rate will ___________.

a)

increase

b)

decrease

c)

stay the same

13.

Which amino acid participate directly in the urea cycle?

a)

Aspartate

b)

Alanine

c)

Fumarate

d)

Glutamine

14.

Graph a is the normal enzyme plot of rate of reaction vs substrate concentration. Graph b and c are the graphs plotted with inhibitors. Graph ____ is a __________ type of inhibition with a lowered Vmax.

a)

b, competitive

b)

b, noncompetitive

c)

c, competitive

d)

c, noncompetitive

15.

Aquatic animals produce ______________ and insects produce _____________ as their nitrogenous waste

a)

ammonia, urea

b)

ammonia, uric acid

c)

urea, ammonia

d)

uric acid, ammonia

16.

In transamination, the NH2 group on one molecule is exchanged with the =O group on the other molecule. The amino acid becomes α-keto acid _____________, and α-keto acid becomes an α-amino acid _____________

a)

(anabolism), (catabolism)

b)

(catabolism), (anabolism)

c)

(anabolism), (biosynthesis)

d)

(catabolism), (breakdown)

17.

Essential amino acids ___________________ .

a)

examples are glutamate and glutamine

b)

needs to be consumed to fulfill our requirements

c)

is already being produced in our body

d)

is needed by E.coli

18.

Production of glutamate is _______________ whereas production of glutamine is _______________.

a)

reductive amination, amidation

b)

reductive amination, amination

c)

reductive amination, transamination

d)

non of above

19.

What is the meaning of these terms: [E], [S], [ES]

a)

Respectively for Enzyme's effectiveness, Substrate, and complex of enzyme-substrate concentration

b)

Respectively for Enzyme, Substrate, and Enzyme-Substrate concentration

c)

Respectively for Enzyme, Substrate and Product concentration

d)

Respectively form Product concentration

20.

 Velocity, V=d[P]dtVelocity,\ V=\frac{d\left[P\right]}{dt}  is the equation for enzyme's velocity. Which of the following information are incorrect? 

a)

Velocity is the rate of product formation over time

b)

Velocity is a measurement of rate

c)

Velocity measures the depletion of the substrate

d)

Velocity measures the formation of the product