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WorksheetsTransgenesis
Total questions: 20
Worksheet time: 13mins
What process is shown in the picture?
Gene therapy
Gene cloning
DNA Fingerprinting
Gene Splicing
Which technology below would probably be the most important to a person who had diabetes and had to take insulin every day?
using recombinant DNA to produce human hormones from bacteria
testing parents for genetic disorders before they have children
engineering fruits and vegetables that produces insulin
developing ways to identify criminals through DNA fingerprinting
The first mammal cloned was a sheep named ............................
Dolly
Doll
Dilla
Dolly Parton
What are the purpose of restriction enzymes during genetic modification?
To cut the DNA of the organism and the plasmid up into small pieces
To stick the DNA back to its original location
To 'cut out' the gene from the DNA, and to cut the plasmid
To put the plasmid into the DNA of the organism
After finding the location of a gene, what enzyme can a scientist use to produce recombinant DNA?
Using an inverted microscope
Using Taq polymerase
Using ligase
Using restriction enzymes
If you want to make a GM crop that is purple, what is the first thing you need to do?
Find a plant that is naturally purple for the gene source
Breed with a purple plant
Use an enzyme to cut a gene out of a normal plant
Use a virus to insert a gene into a plant
How does transgenesis work?
A gene from one organism is inserted into the genome of another organism.
Organisms with a desirable trait are breed together until offspring show improved trait
Gene are silenced by harnessing our bodies natural defense system against viruses.
An organism is reproduced by removing the nucleus from one of its cells and inserting that nucleus into an egg, which is then develops in the host womb.
What biotechnology technique was able to produce this glowing cat?
Transgenesis
Biolistic
Selective Breeding
RNAi
What is a the basis for genetically modifying livestock?
Produce more meat
Produce shiny fur
Produce colorful fur
Produce more fat
An argument in favor of food biotechnology is that it
provides foods that cure viral diseases.
provides more food to feed a growing population.
provides a cash crop for low income nations.
biotech foods are not controversial
What happens in Step 4?
Rectriction Enzyme, EcoRI, cuts the plasmid and the insert
Insert is being replicated in host cell
Recombinant DNA is transformed in bacteria
Selected gene from foreign DNA is inserted into plasmid by using ligase
Which is the use of genetically modified bacteria?
to be the host for recombinant cosmids.
develop DNA library
making human insulin for diabetics
producing corn that is resistant to herbicides
All of the following are physical methods of gene transfer except
Electroporation
Transduction
Microinjection
Biolistic
Bt gene is got from
Brassica napus
Bacillus thuringiensis
Burkholderia thailandensis
Bacteroides termitidis
Microparticles for coating with DNA to be bombarded with gene gun are made of
Gold or tungsten
Platinum or zinc
Silver or platinum
Silicon or platinum
What is true of Bt toxin?
The concerned bacillus has antitoxins
The inactive protoxin get converted into active form in the insect gut
Bt protein exists as active toxin in bacillus
The activated toxin enters the ovaries of the pest to sterilize it an thus prevents its multiplication
The pathogen of plant roots that can cause crown gall disease is
Rhizobium
Agrobacterium tumefaciens
Escherichia coli
Saccharomyces cerevisiae
Direct uptake of exogenous DNA through the cell membrane by a competent cell is called
Conjugation method
Bacterial transformation
Retroviral transduction
Agrobacterium mediated transfer
In liposome mediated gene transfer, foreign DNA entry route is via ____________ whereas in non-liposomal mediated gene transfer, gene entry is via _______________ process.
endocytosis, phagocytosis
phagocytosis, endocytosis
exocytosis, phagocytosis
phagocytosis, exocytosis
