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WorksheetsGene Cloning
Total questions: 20
Worksheet time: 10mins
Restriction enzymes are analogous to what common school supply?
glue
scissors
paper
highlighter
Restriction Enzymes can cut at any sequence of DNA
True
False
The site where restriction enzymes cut are a called:
cut sites
restriction sites
recognition sites
cleave sites
Where are restriction enzymes naturally found?
in humans
in bacteria
in algae
they are not natural - all restriction enzymes are made in the lab
What is a plasmid?
a section of the human genome
a material that cuts DNA
viral DNA
an accessory piece of DNA found in bacteria
If a linear piece of DNA contains 3 recognition sites for a restriction enzyme. How many pieces will be produced when the restriction enzyme cuts the DNA?
2
3
4
5
6
Sticky ends are produced when the restriction enzyme cuts in a:
staggered manner
straight through the DNA
A plasmid is larger than a bacterial genome
True
False
Plasmids often have genes that give the bacterial a survival advantage
True
False
The multiple cloning site in the plasmid only allows one restriction enzyme to be able to cut open the plasmid
True
False
What enzyme binds to the promotor of the plasmid to transcribe the gene inserted into the plasmid?
DNA polymerase
helicase
RNA polymerase
ligase
How many times do you want a restriction enzyme to cut a human genome in order to cut out the gene of interest to insert into a plasmid?
1 - through the gene
2 - on either side of the gene
3 - through the gene and on either side of the gene
4 - twice on each side of the gene
What enzyme is used to ensure that the recombinant DNA created is bonded together along the sugar-phosphate backbone?
DNA polymerase
RNA polymerase
ligase
helicase
Biological cloning is frequently used to make which of the following:
Human growth hormone
Human insulin
genetically modified oil eating microbes
pest resistant crops
all of the options
Chemical cloning uses a machine called a thermocycler to clone the gene
True
False
Each cycle of the thermocycler doubles the number of DNA copies. How many cycles of the thermocycler are needed to make 16 copies of DNA?
2
3
4
5
6
Why are primers needed for the PCR process?
they are what makes copy of the DNA
they are used to build more copies of the DNA
they attach to sites on the DNA on either side of the DNA to be copied
they are what change temperature to copy the DNA
Why is DNA polymerase needed in the PCR process?
they are the building blocks to make more DNA
it is used as a template to make more DNA
it attaches to either end of the gene to be copied
it is what makes a complementary copy of the template DNA strand
Why are nucleotides needed in the PCR process?
they are the building blocks used to build the copies of DNA
they are the template that is used to make copies of the DNA
they are the machine that changes temperature to make DNA copies
they attach to either end of the DNA that needs to be copied
Why is the first heating up stage needed in the PCR cycle?
it allows the primers to attach the DNA
it allows the DNA polymerase to attach to the DNA to begin copying it
it attaches the nucleotides to make DNA
it unwinds the double stranded DNA so both strands can be used as a template to make more DNA
