WorksheetsMicrobial Biodiversity
Total questions: 15
Worksheet time: 24mins
If the iodine step were omitted in the Gram-staining procedure, what color would you expect Gram-positive bacteria to stain and what color would you expect Gram-negative bacteria to stain?
Gram-positive = purple; Gram-negative = red
Gram-positive = purple; Gram-negative = purple
Gram-positive = purple; Gram-negative = colorless
Gram-positive = red; Gram-negative = red
Choose the best microscope to visualize relative species abundance in microbial communities
combination of microscopy and species-specific oligonucleotide hybridization probes
combination microscopic techniques and spectrophotometric assays with X-ray fluorescence
confocal laser scanning microscopy
backscattered scanning electron microscopy
Bacterial physiology differs from one type of organism to another. Base on this statement bacterium can be identify using
Biochemical Test
16s rRNA
Morphology observation
Riboprinter analysis
One way to determine the size, cell morphology and grouping of bacterial cells is to use a __________ technique.
streak plate
phenol red
simple stain
nutrient broth culture
Choose the correct comparison between catabolism and anabolism
Catabolism is synthesis processes whereas anabolism degrade compounds
Catabolism is exergonic whereas anabolism endergonic in nature
Catabolism produce macromolecules whereas anabolism produce small molecules
Both processes do not link to each other
Enzymes work by which of the following?
increasing the activation energy
reducing the activation energy
making exergonic reactions endergonic
making endergonic reactions exergonic
The term apoenzyme is applicable to
Simple enzyme
Protein part of conjugate enzyme
Organic cofactor of a conjugate enzyme
Inorganic cofactor of a conjugate enzyme
Bacillus sp was grown on starch agar. After 24 hours of incubation, iodine has been added to the plate and clearing zone can be observing around the colony. Explain how it could be
(a)
In a viable plate count, each ___represents a ___from the sample population.
cell, colony
colony, cell
hour, generation
cell, generation
Toxic forms of oxygen are broken down by several enzymes in obligate aerobes bacteria. List all the possible enzyme
(a)
Identify the correct metabolic event related to TCA (Krebs) cycle
H+ and e- are delivered to O2 as the final acceptor
Pyruvic acid is formed
NADH+ is oxidized
CO2 is formed
Identify the correct metabolic event related to electron transport/oxidative phosphorylation
GTP is formed
Pyruvic acid is formed
ATP synthase is active
CO2 is formed
Incomplete oxidation of glucose into pyruvic acid with several intermediate steps is known as
TCA – pathway
Glycolysis
HMS-pathway
Krebs cycle
Krebs cycle forms an important product
Acetyl CoA
ADP
ATP
Water
Metabolic water is the one that is
Used during photosynthesis
Produced during polymerisation
Produced during transamination
Formed during aerobic utilization of glucose
