Font size
WorksheetsGene and cDNA libraries
Total questions: 10
Worksheet time: 8mins
Types of gene libraries (pick more than one answers)
cDNA
mRNA
Genomic
All of the following
What is a cDNA?
collection of total genomic DNA
non-coding RNA molecule (containing about 22 nucleotides) found in plants,
complementary DNA molecules synthesized from mRNA
small interfering Ribonucleic Acid, is a class of double-stranded RNA molecules
A gene library is a collection of restriction enzymes where each contain a portion of the genetic material of interest .
True
False
Where do we insert vectors?
bacterial host cells
restriction enzymes
culture media
a and b only
Below are correct statements regarding a probe EXCEPT
A probe is used to search for its complementary sequence in a sample genome.
The probe is labeled with a radioactive or chemical tag that allows its binding to be visualized
A probe is a single-stranded sequence of DNA or RNA
A probe is a double-stranded sequence of DNA or RNA
In the formation of a cDNA library, RNase used is
RNAse H
RNAse A
The diagram showed the steps in creating genomic library. Step 1 starts with genomic DNA extraction. Step 2 that followed would be
Transformation into appropriate vecors
Partial digestion with frequently used 4 base cutters
The diagram showed the steps in genomic library creation. The last step is screening for positive clones. Screening includes all the methods below EXCEPT
colony hybridization screening with a probe
screening on a substrate specific medium
screening with polymerase chain reaction (PCR)
screening using Western blotting
A genomic library can be built in a
phage library form
plasmid library form
YACs library form
All of above
Below are components/enzymes used in cDNA library construction EXCEPT
using isolated cellular mRNA
using genomic DNA
using the cell's transriptome
using reverse transcriptase
