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DNA structure and replication

Total questions: 20

Worksheet time: 28mins

Name
Class
Date
1.

Which 2 molecules form the sides (backbone) of the DNA ladder?

a)

deoxyribose sugar and purine

b)

deoxyribose sugar and a hydrogen bond

c)

deoxyribose sugar and pyrimidine

d)

deoxyribose sugar and phosphate

2.
Nitrogenous bases are joined by which type of bond?
a)
polar bonds
b)
ionic bonds
c)
covalent bonds
d)
hydrogen bonds
3.
The enzyme that unzips the DNA to prepare for replication
a)
helicase
b)
replicase
c)
polymerase
d)
synthase
4.
If a strand reads:
3' A A A T T T C 5'
The matching strand would read...
a)
5' T T T A A A G 3'
b)
3' T T T A A A G 5'
c)
5' A A A T T T C 3'
d)
3' U U U A A A G 5'
5.
Polymerase is able to replicate the new DNA ______ along the leading strand.
a)
continuously
b)
only in short fragments
c)
very slowly
d)
prokaryotically
6.
These are the "smaller", single ringed bases such as thymine and cytosine
a)
complementary
b)
 cytosine
c)
purine
d)
pyrimidine
7.
These are the "larger", double-ringed bases such as adenine and guanine.
a)
complementary
b)
 cytosine
c)
purine
d)
pyrimidine
8.

Which enzyme is responsible for adding the RNA primer to the template strand?

a)

Helicase

b)

DNA Polymerase

c)

Ligase

d)

Primase

9.
Which sequence of DNA bases would pair with this partial strand
ATG TGA CAG
a)
ATG TGA CAG
b)
TAC ACT GTC
c)
GTA AGT GAC
d)
CAT TCA CTG
10.
Why does DNA replicate?
a)
To create another copy for the new cell
b)
To create a duplicate copy in case the original gets damaged
c)
To create a new copy to replace the damaged original
11.
What are the gaps between the newly synthesized DNA on the lagging strand called?
a)
Okazaki fragments
b)
Polymer gaps
c)
Helicase fragments
d)
Acidic gaps
12.

Which enzyme joints the Okazaki fragments in the lagging strand?

a)

Helicase

b)

Polymerase

c)

Ligase

d)

Primerase

13.

What did Meselson and Stahl conclude?

a)

DNA is conservative. Radioactive strand forms nonradioactive strand. The nonradioactive strands bind to themselves and the radioactive strands bind to themselves

b)

DNA is semiconservative. You see two bands in the R2 stage. Middle band is half radioactive, half nonradiaoctive.

c)

DNA is dispersive. Some radioactiveness spreads to the other strands. Thus, in R2, you would only have one density middle band.

d)

DNA undergo conservative replication for the first generation, but follow semiconservative replication in the following generations.

14.
What enzyme is involved in relieving tension during unwinding?
a)
Topoisomerase
b)
Helicase
c)
Ligase
d)
Single Strand Binding Protein
15.

What is the correct order for DNA replication?

a)

Unwinding, Initiation, Termination

b)

Unwinding, Initiation, Elongation, Termination

c)

Unwinding, elongation, initiation, termination

d)

Initiation, unwinding, elongation, termination

16.

What is the difference between DNA polymerase III and DNA polymerase I?

a)

DNA P1 replaces the RNA primer with DNA nucleotides, leaving ligase to bind the phosophodiester bonds

b)

DNA P1 recognizes the RNA primer, extends the chain, and proofreads sequences

c)

DNA P3 replaces the RNa primer with DNA nucleotides, leaving ligase to bind the phosphodiester bonds

d)

DNA P3 works together with DNA P2

17.

Function of helicase is

a)

Unwind DNA double strand

b)

Relieve strain of DNA strand

c)

Add free DNA nucleotide

d)

Forming lagging strand

18.

RNA differs from DNA in all of the following except:

a)

RNA is a single strand instead of a double strand.

b)

Uracil replaces Thymine as a nitrogenous base.

c)

Ribose sugar is used instead of deoxyribose.

d)

RNA is antiparallel to DNA.

19.

The point at which two strands of a DNA molecule split is called:

a)

Origin of replication

b)

Replicated nucleotides

c)

Antiparallel strands

d)

Replication fork

20.

What are antiparallel DNA strands?

a)

DNA strands that run opposite to each other

b)

DNA strands that run in the same direction

c)

DNA strands that contain the same nucleotides

d)

DNA strands that need repaired