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WorksheetsGenetic Engineering Class test
Total questions: 10
Worksheet time: 5mins
Restriction enzymes were first discovered in E.coli restricting the replication of ...................., by cutting the DNA
Yeast
Bacteriophages
E.coli
lambda
•DNA ligase joins (seals) the DNA fragments by forming a .......................... bond between the phosphate group of 5′-carbon of one deoxyribose with the hydroxyl group 3′-carbon of another deoxyribose
Phosphodiester
Hydrogen
Weak
all the above
Alkaline phosphatase is an enzyme involved in the ...................phosphate groups.
addition of
removal of
both addition and removal
none of the above
pBR322 is ................base pairs in length
4356
4359
4361
4369
Identify the vector
pBR 322
PUC18
Cosmid
phagemid
........................library is made by cloning DNA made in vitro by reverse transcription of all the mRNA produced by a particular cell
Genomic
cDNA
Both
none of the above
A clone carrying the gene of interest can be identified with a ....................... having a sequence complementary to the gene
primer
isotopes
lacZ
nucleic acid probe
Northern blotting technique was developed by
Neal Burnette
Towbin's group
James alwin
George Stark's group
Who is the father of biotechnology?
Identify the technique
Southern Blotting
Northern Blotting
Western Blotting
Insitu Hybridization
