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Biophysics Test 1

Total questions: 20

Worksheet time: 19mins

Name
Class
Date
1.
Complete the sentence.
A substance that is more basic has a _____ pH than a substance that is acidic.
a)
lower
b)
higher
c)
the same
d)
none of the above
2.

Water has a neutral because

a)

it has more H+ ions than OH-

b)

it has more OH- ions than H+

c)

it does not produce any ions

d)

it has an equal amount of H+ and OH- in solution

3.
A high Rvalue indicates strong
a)
strong affinity to the stationary phase
b)
strong affinity to the mobile phase
c)
no affinity to the stationary phase
d)
 no affinity to the stationary phase
4.
chromatography separates the mixture of dyes on the basis of their ----------------------------
a)
density
b)
solubility
c)
gravity
d)
boiling point
5.
The mobile phase in paper chromatography is typically a
a)
plasma
b)
solid
c)
gas
d)
liquid
6.
The substance in the mixture that has the greatest affinity for the stationary phase will travel
a)
fastest
b)
slowest
7.

In case of chromatography the solvent rises up due to ……………………..

a)

Solute

b)

Filter paper

c)

Capillary action

d)

None of the above

8.

Which of the following substances can be separated using chromatography?

a)

Amino acids

b)

Ink

c)

Sugars from starch

d)

All the above

9.

The amount of light absorbed by a sample is called its:

a)

Absorbance

b)

Transmittance

c)

Hue

d)

Saturation

10.

Which of the following statements is correct??

a)

Spectrophotometry allows for a correlation to be made between samples

b)

Spectrophotometry allows for a correlation to be made between patient absorbance

c)

Spectrophotometry allows for a correlation to be made between absorbance and concentration

d)

Spectrophotometry allows for a correlation to be made between the concentration of each sample

11.

This technique would be employed when a rapid diagnosis is necessary:

a)

Serial sections

b)

Levels

c)

Routine paraffin section

d)

Frozen section

12.

When you focus on a new specimen, always begin with

a)

low power and coarse adjustment

b)

low power and fine adjustment

c)

high power and coarse adjustment

d)

high power and fine adjustment

13.
If your teacher asks you to move a microscope to table, how do you carry it?
a)
by the body tube and the eyepiece
b)
with one hand
c)
one hand on the arm and the other under the base
d)
with one hand on the base and the other on the eyepiece.
14.

Total magnification is:

a)

the strength of the eyepiece lens

b)

the strength of the eyepiece multiplied by the objective lense

c)

using a magnifying glass

d)

what you see under the objective lens

15.

Which among the following has the highest magnification power?

a)

Magnifying lens

b)

Compound Microscope

c)

TEM

d)

SEM

16.
What is needed to produce an image for an electron microscope?
a)
Light
b)
A living cell
c)
A vacumn
d)
Favorable weather
17.

Explain the principle of phase contrast microscope? (3 Marks)

4 lines
18.

Explain different types of centrifugation. (3 Marks)

4 lines
19.

Discuss the working of fluorescence microscopy. (3 Marks)

4 lines
20.

Comment on different types of microtome. (5 Marks)

4 lines