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WorksheetsBIOTECHNOLOGY, QUIZ 1
Total questions: 10
Worksheet time: 5mins
Gene cloning is:
Gene is isolated and inserted in same organism
Gene is isolated and inserted in different organism
Gene is isolated and inserted in plasmid of other organism
Gene is isolated and inserted in chromosomal DNA
Chimeric DNA is
Gene clone
Recombinant-DNA
Transposon
Vector shuttle
In Ti-plasmid, which of the following is removed:
Auxin gene
Virulent gene
Cytokinin gene
Auxin & cytokinin gene
The two antibiotic resistance genes on vector pBR322 are for
Tetracycline and Kanamycin
Ampicillin and Tetracycline
Ampicillin and Chloramphenicol
Chloramphenicol and Tetracycline
Which one of the following equipments is essentially required for growing microbes on a large scale, for industrial production of enzymes?
BOD incubator
Sludge digester
Industrial oven
Bioreactor
DNA precipitation out of a mixture of biomolecules can be achieved by treatment with
Isopropanol
Chilled ethanol
Methanol at room temperature
Chilled chloroform
Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement.
The enzyme cuts DNA molecule at identified position within the DNA.
The enzyme binds DNA at specific sites and cuts only one of the two strands.
The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.
The enzyme recognizes a specific palindromic nucleotide sequence in the DNA.
A selectable marker is used to :
Mark a gene on a chromosome for isolation using restriction enzyme
Help in eliminating the non-transformants, so that the transformants can be regenerated
Identify the gene for a desired trait in an alien organism
Select a suitable vector for transformation in a specific crop
In PCR, it is _________ that creates single-stranded template molecules
heat
high salt concentration
DNA polymerase
exonuclease
Ideally, PCR __________ increases the amount of DNA during additional cycles
additively
gradually
linearly or systematically
exponentially
