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BIOTECHNOLOGY, QUIZ 2

Total questions: 10

Worksheet time: 5mins

Name
Class
Date
1.

The uptake of naked DNA by bacteria is called :

a)

Conjugation

b)

Transformation

c)

Transfection

d)

Transduction

2.

118. Isolation of DNA from a fungal cell involves the use of enzyme

a)

Chitinase

b)

Lysozyme

c)

Eco R1

d)

Hind-II

3.

Most commonly used matrix in gel electrophoresis is

(a) (b) (c) (d)

a)

ethidium bromide

b)

agarose

c)

polyacrylamide

d)

sulphate bromide.

4.

Which of the following is common in sticky ends and molecular probes?

a)

They both are parts of RNA molecules.

b)

They both involve complementary pairing.

c)

They both are important aspects of bacterial sex.

d)

They both are used as gene vectors in genetic engineering.

5.

During “gene cloning” which is called a gene taxi?

a)

Vaccine

b)

Plasmid

c)

Bacteria

d)

Protozoa

6.

Which of the following chemicals is used in vectorless gene transfer?

a)

Polyethylene glycol

b)

Ethidium bromide

c)

Ethidium sulphide

d)

Sodium bromide

7.

The process of separation and purification of expressed protein before marketing is called :

a)

Downstream processing

b)

Bioprocessing

c)

Postproduction processing

d)

Upstream processing

8.

For a DNA to function as a cloning vector the most essential requirement is -

a)

multiple restriction sites

b)

several selectable markers

c)

circular nature

d)

'ori' sequence

9.

In “QB Bacteriophase” genetic material is :

a)

DNA

b)

RNA

c)

Protein

d)

None

10.

Which of the following is not used in polymerase chain reaction?

a)

Taq polymerase

b)

dNTPs

c)

Primers

d)

Ca2+