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LAB - How big do you know micro?

Total questions: 35

Worksheet time: 6mins

Name
Class
Date
1.

The positive control organism used in the Acetamide Test.

a)

Stenotrophomonas maltophilia

b)

Escherichia coli

c)

Staphylococcus aureus

d)

Pseudomonas aeruginosa

e)

Vibrio cholerae

2.

What color is the positive result of Acetate Utilization Test?

a)

Red

b)

Yellow

c)

Green

d)

Blue

e)

Purple

3.

This organism is used as the positive control in the Acetate utilization test.

a)

K. pneumoniae

b)

E.coli

c)

P. aeruginosa

d)

P. vulgaris

e)

S. pyogenes

4.

What color is the positive result of Acetamide Test?

a)

Red

b)

Green

c)

Blue

d)

Purple

e)

Yellow

5.

What specific end product is produced when the amino acid, ornithine, undergo decarboxylation?

a)

arginine

b)

putrescine

c)

cadaverine

d)

phenylalanine

e)

tryptophan

6.

What specific end product is produced when lysine undergo decarboxylation?

a)

cadaverine

b)

arginine

c)

tryptophan

d)

putrescine

e)

phenylalanine

7.

The indicator used in the the Amino Acid Decarboxylase Test?

a)

ninhydrin

b)

bromthymol blue

c)

phenol red

d)

bromcresol purple

e)

emerald green

8.

What is the end product of the bile esculin test?

a)

Esculetin

b)

Bile salt

c)

Hydolyzed bile salt

d)

Esculin

e)

None

9.

The organism being detected in the bile esculin test.

a)

E. rhusiopathiae

b)

S. agalactiae

c)

K. pneumoniae

d)

E. faecalis

e)

None

10.

This biochemical test may be used to differentiate Streptococcus pneumoniae from Streptococcus mitis.

a)

Bile esculin test

b)

Bile solubility

c)

CAMP test

d)

PYR hydrolysis test

e)

None

11.

Which organism will lyse bile salt in the Bile Solubility Test.

a)

S. agalactiae

b)

S. pneumoniae

c)

S. pyogenes

d)

S. bovis

e)

None

12.

This biochemical test is used in the presumptive identification of Moraxella catarrhalis.

a)

LAP test

b)

beta lactamase test

c)

acetamide utilization test

d)

butyrate disk test

e)

None

13.

Cetrimide when added into a culture medium results into a selective medium recommended for use in qualitative procedures for isolation and presumptive identification of what organism?

a)

E.coli

b)

K. pneumoniae

c)

V. cholerae

d)

P.aeruginosa

e)

S. pneumoniae

14.

The positive result/color of the biochemical test Citrate Utilization Test.

a)

purple

b)

hemolysis

c)

blue

d)

clearing

e)

red

15.

Which of the following organisms will mostly test positive or liquefy gelatin?

a)

E. cloacae

b)

E.coli

c)

P. rettgeri

d)

S. marcescens

e)

S. boydii

16.

Which will be able to grow at 42OC.

a)

P. vulgaris

b)

S. maltophila

c)

S. paratyphi

d)

S. marcescens

e)

P. aeruginosa

17.

Indicator used in hippurate hydrolysis test

a)

ferric ammonium sulfate

b)

beta-lactamase

c)

hippuricase

d)

ninhydrin

e)

bromcresol purple

18.

All of the following biochemical test will produce H2S as end product, except for:

a)

Krigler's Iron Agar

b)

Simmons' citrate agar

c)

Lysine decarboxylase

d)

Triple sugar iron

e)

SIM Media

19.

Organisms that grow as “puff balls” in thioglycollate broth.

a)

Bacilli

b)

Cocci

c)

Spirilli

d)

Pleomorphic

e)

Vibrio

20.

Bacterial growth is indicated by a change in the ordinary broth’s appearance from:

a)

Turbidity

b)

Clearing

c)

Change in color

d)

Movement from the stabbed area

e)

Growth in streaked area

21.

In Bacitracin susceptibility testing, Micrococci spp. produce zone of inhibition against the antibiotic bacitracin.

a)

TRUE

b)

FALSE

22.

This test is used for the presumptive identification and differentiation of Streptococcus pyogenes.

a)

CAMP

b)

Bile esculin

c)

Bacitracin

d)

Optochin

e)

Hippurate hydrolysis

23.

This particular test is very helpful in distinguishing Streptococcus agalactiae from other Streptococci

a)

Bacitracin

b)

Optochin

c)

Bile solubility

d)

Bile esculin

e)

CAMP

24.

What known organism should be used in able to perform the CAMP Test?

a)

S. aureus

b)

S. agalactiae

c)

L. monocytogenes

d)

C. diphtheriae

e)

R. equi

25.

This test makes use of antibiotic paper disk to exhibit zone of inhibition as its positive result.

a)

Oxidase

b)

Optochin

c)

LAP

d)

Coagulase

e)

Catalase

26.

This Haemophilus sp. only requires V Factor for its growth

a)

Haemophilus ducreyi

b)

Haemophilus aprophilus

c)

Haemophilus influenzae

d)

Haemophilus parahaemolyticus

e)

Haemophilus stutzeri

27.

The X Factor disk used in identifying Haemophilus are paper disk impregnated with nicotinamide adenine dinucleotide.

a)

TRUE

b)

FALSE

28.

The general positive result of this test either from different method used is the development of a deep purple/blue color.

a)

CAMP

b)

Indole

c)

Oxidase

d)

Optochin

e)

Catalase

29.

It is termed as the lowest concentration of the antimicrobial agent that inhibits growth of the organism.

a)

MBC

b)

MIC

c)

Susceptibility testing

d)

Resistant

e)

Intermediate

30.

When the bacterium respond to the usual doses of the drug it is interpreted as _________________:

a)

Susceptible

b)

Resistant

c)

Intermediate

d)

MBC

e)

Synergy

31.

What is the very minor error in suscpetibility testing?

a)

Intermediate results

b)

Resistant becomes susceptible

c)

Susceptible becomes resistant

d)

Equivocal results

e)

None

32.

Concentration of antibiotic in the serum is termed as ____________.

a)

Dose for inhibition

b)

McFarland Standard

c)

MIC

d)

Antibiotic level

e)

Synergy

33.

The following antibiotics are cell wall inhibitors, EXCEPT:

a)

Polymyxin B

b)

Meropenem

c)

Oxacillin

d)

None

34.

The following are protein synthesis inhibitors, EXCEPT:

a)

Pristinamycin

b)

Dalfopristin

c)

Quinupristin

d)

Oxacillin

e)

None

35.

The preliminary identification of microorganism is usually done by:

a)

Isolating in a culture media

b)

Susceptibility testing

c)

Biochemical testing

d)

Gram staining

e)

None among the choices