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Worksheetscultural media
Total questions: 24
Worksheet time: 12mins
Most microorganisms pathogenic for humans grow best in the laboratory when cultures are incubated at
15–20°C
20–30°C
30–37°C
38–50°C
50–55°C
The process by which microorganisms form ATP during the fermentation of glucose is characterized by
Coupling of ATP production with the transfer of electrons
Denitrification
The reduction of oxygen
Substratephosphorylation
Anaerobic respiration
The major effect of a temperature of 60oC on the growth of a mesophile such as Escherichia coli is to
Destroy the cell wall
Denature proteins
Destroy nucleic acids
Solubilize the cytoplasmic membrane
Cause formation of endospores
Polymerization of building blocks (eg, amino acids) into macromolecules (eg, proteins) is achieved largely by
Dehydration
Reduction
Oxidation
Assimilation
Hydrolysis
A strain of E coli does not require vitamins when grown in a defined medium consisting of glucose, mineral salts, and ammonium chloride. This is because E coli
Does not use vitamins for growth
Obtains vitamins from its human host
Is a chemoheterotroph
Can synthesize vitamins from the simple compounds pro-
vided in the medium
Ammonium chloride and mineral salts contain trace
amounts of vitamins
Which of the following is NOT a mechanism for generating metabolic energy by microorganisms?
Fermentation
Protein synthesis
Respiration
Photosynthesis
C and D
Which of the following terms best describes a microorganisms that grows at 20°C?
Neutralophile
Psychrotroph
Mesophile
Osmophile
Thermophile
The ability to assimilate N2 reductively via NH3 is called
Ammonification
Anammox
Assimilatory nitrate reduction
Deamination
Nitrogen fixation
Which of the following is NOT assimilated by eukaryotic cells?
Glucose
Lactate
Sulfate (SO42-)
Nitrogen(N2)
Phosphate (PO43-)
Bacteria that are obligate intracellular pathogens of humans (eg, Chlamydia trachomatis) are considered to be
Autotrophs
Photosynthetic
Chemolithotrophs
Hyperthermophiles
Heterotrophs
Which of the following is an enrichment media
Nutrient broth
Tetrathionate broth
Stuart medium
Thayer-martin medium
Which of the following statements is true
Agar has nutrient properties
Chocolate agar is a selective medium
Addition of selective substances in a solid medium is called enrichment media
Nutrient broth is a basal medium
MacConkey agar is an example of
Differential medium
Transport medium
Enriched medium
Anaerobic medium
All of the following are enriched media except
Loefflers serum slope
Blood agar
Chocolate agar
Nutrient agar
A solid medium containing substances which allows wanted bacteria to grow inhibits the growth of unwanted bacteria is called
Differential medium
Enriched medium
Enrichment medium
Selective medium
Stuarts transport medium is used for the transport of specimens containing
Salmonellae
Gonococci
Vibrio cholera
Shigellae
Robertson’s cooked meat medium is used to grow
Anaerobes
Viruses
Yeasts
Aerobes
Agar-agar is used in the microbiological media for
Nutritive value
Solidifying media
Carbon source
All of the above
Which of the following selective media is used to isolate M.tuberculosis
Deoxycholate citrate medium
Thayer –martin medium
Lowenstein-jensen medium
Potassium tellurite blood agar
All of the following are true about sabourauds dextrose agar except
Contains glucose
Has alkaline PH
Selective medium for fungi
Contains cycloheximide
Mcintosh –fildes anaerobic jar is used for the isolation of
Clostridium tetani
Pseudomonas aeruginenusa
Streptococcus pyogenes
None of the above
Lawn culture method is used for
Antibiotic sensitivity testing
Bacteriophage typing
Preparation of bacterial antigen
All of the above
The growth of bacteria in liquid culture medium is indicated by the presence of
Turbidity
Deposit
Surface pellicle
All of the above
Triple sugar iron medium contains the following sugars except
Glucose
Sucrose
Lactose
Maltose
